Study On Determination Of Trace Amounts Of Environmental Hormone In Surface Water Samples

Environmental hormone is a series of chemical compounds in environment. It may affect human and wildlifes on their normal endocrines, cause reproductive, developmental and behavioral disorder. In this paper, three kinds of environmental hormones such as phthalic acid esters(PAEs), phenols and the estrogen materials were choosed as the research object. The analytical methods of three environmental hormones have been improved and perfected in this study. The study not noly provides effective and accurate detection means of environmental hormone in practical water samples, but also can help to master the pollution present condition, distribution rules, migration and transformation of environmental hormone in water environment.Four of phthalic acid esters (DEP, DBP, DCHP, DEHP) have been studied in this paper. Solid phase extraction (SPE) combining with gas chromatography(GC) was used to determine PAEs. Four primary factors which may affect recoveries of analytes were optimized by orthogonal design. The optimized results were as follows: eluting solvent was dichloromethane, eluting rate was 2mL/min, amount of eluting solvent was 4mL, flow-rate was 5mL/min. Linear relativity of four PAEs were better and correlation coefficient were about 0.99, the linear range of 4 PAEs were wide and all can reach 1-5mg/L, and the determination limits of 4 PAEs were 0.06μg/L. The precision and accuracy were studied, recoveries of 4 PAEs were between 70% and 102%, relative standard deviations (RSD) were less than 7.6%.As for higher polar environment hormones(bisphenol A, 4-nonylphenol, pentachlorophenol, 2,4-dichlorophcnol, estradiol, estriol, 17α-ethinylestradiol, estmne), which always called for derivation in the process of pretreatment. Therefore, the analytical method contained three parts as SPE, derivation and GC. Derivation experiment has been designed by considering main factors which may affect derivation. The optimal derivation conditions were chosen as ( BSTFA100μL, 60℃, 0.5h ). Orthogonal design was made under the optimal derivation conditions, The results were as follows: the eluting solvent was 6mL dichloromethane with the elution rate 0.5mL/min and pH 6.42. Furthermore, the experimental data appeared perfect Linear relativity of 8 phenols and estrogen materialsand correlation coefficient can reach above 0.99, all of the linear of 8 phenols and estrogen materials ranged from 5 to 50mg/L, and the determination limit of 8 phenols and estrogen materials were 1μg/L except 17α-ethinylestradiol. The precision and accuracy were studied, recoveries of 8 phenols and estrogen materials were between 80% and 115%, and relative standard deviations (RSD) were between 5.5% and 8.0%.The influent of the water plant has been chosen to be tested the concentration of environmental hormones. The results followed as: the source of the water plant has been polluted by PAEs, of which DBP has the highest concentration and reached 28.26μg/L; Moreover, some phenols and estrogen materials have been found, the concentration of 4-nonylphenol and 17α-ethinylestradiol were higher than others with concentration as 0.86μg/L and 0.50μg/L, respectivly. Nevertheless, the contaminated situation of phenols and estrogen materials are not more serious than PAEs.