Preparation Of Magnetic Polymer Spheres And Their Applications In The Resolution Of Amino Acids By Immobilized Aminoacylase

Amino acids are the basic building blocks for proteins and nutritionally important key compounds. They play significant roles in the life. Among 20 basic amino acids, glycine is the only one that does not exhibit chirality, and the other 19 are all L-amino acids. With the development of amino acids industry, the functions of D-amino acids are being discovered. Resolution by aminoacylase is one of the best methods in the resolution of amino acids. It is a high efficient, green method.Magnetic polymer microspheres, due to their special properties of polymer microspheres and rapid magnetic separation, have been widely applied to various aspects in biotechnology field.In this paper, aminoacylase is covalently immobilized onto the magnetite particles modified by 3-aminopropyltrie-thoxysilane supports via glutaric dialdehyde. And we use this immobilized enzyme to chirally split the Amino acids. Through SEM, TEM, XRD, and FT-IR analysis, the structure of magnetite particles supports modified by 3-aminopropyltrie-thoxysilane are characterized.Through an enzyme activity analysis, it proved that the modified magnetite supports could improve the stability and the recycle of the enzyme.