Studies On Solid-Phase Synthesis, Purification And Preparation Of Peptide JFT

With the development of the living standard,obesity has been an worldwide chronic disease following lots of complications which was very harmful for hunman healthy.More and more medicines for reducing weight have been researched out with deeply cognition about the mechanism of regulating weight.Some of the medicines have did animal experiments and had a good treatment result,some researches were on the molecular level.The medicine which have a good treatment result and little side effects are more and more popular, results of the expements are showing a nice future on reducing weight healthly to us.The peptide JFT in this thesis is sieved by the bank of peptide which can increase energy consumed, and stimulate our body to change the energy to the form of heat. This peptide with good medical value has a large masket.The aim of this thesis is finding a optimized solid-phase peptide synthesis method which can also reduce the cost.Solid-phase peptide synthesis method is used in this thesis,JFT peptide was synthesized successgully at first time with the raw material of FMOC-amino acid and FMOC Rink-Amide MBHA resin as the vector.We discussed the influence to the finall result of synthesis by choosing combination,deblock and cleavage reagents. The combination rate was increased through the ways as fllows:blccking the active regiment of the resin after the first amino acid connecting on it,blocking the active regiment of the amino acid which is difficult to elongate,conjucting a temporary regiment to change the conformation of the peptide chain which can be cleaved easily. We have a replacement of D-amino acid which can make the peptide more active and stablly.It was identified by MALDI—MS that the synthesized peptide have the same mass weight with the theoretical one.The raw peptide was separated and purified by RP-HPLC.We got the product with the purity of 98.12%,and the finall yield was 56.4 %.This synthesis-separation method is simple and convenient which have a short cycle and a low cost to amplify.