Application Of Fluorescence And Resonance Rayleigh Scattering In Determination Of Some Organic Molecules And Physicochemical Parameters

Molecular Spectrometry is a technique widely used in environment science, medicine science, agriculture science, and cosmic science. It has attracted our attention for its remarkable characteristic of high sensitivity, good selectivity and simple operation. This paper studied the application of molecular Spectrometry (fluorescence Spectrometry and resonance Rayleigh scattering Spectrometry) in the determination of some organic molecules and calculation of some physicochemical parameters. It contains three parts:Part 1. The principle and application of fluorescence Spectrometry and resonance Rayleigh scattering Spectrometry in analytical chemistry were summarized. The development of boronic acid receptor and the principle of its interaction with saccharides were introduced.Part 2. The concentration of ferulic acid in traditional Chinese medicine was detected using fluorescence Spectrometry.The fluorescent characteristic was studied. Ferulic acid could emit strong fluorescence in Britton Robison buffer solution at pH 7.0 and the change of fluorescence intensity is related with the concentration of ferulic acid. The present method allowed the determination of ferulic acid in the concentration range of 3.0×10^-7 to 5.0×10^-6 mol/L. The relative standard deviation was 2.1 % for 10 replicate analyses of 1.0×10^-6 mol/L ferulic acid and the detection limit for ferulic acid was 9×10^-9 mol/L. The proposed method was applied to the determination of ferulic acid in Chinese DangGui with satisfactory results.Part 3. The interaction of boronic acid and saccharides was studied. Boronic acid could combine with 1, 2 or 1, 3-diols to form five or six-membered rings respectively via two covalent bonds. This characteristic was applied in the determination of mono-saccharides and some physicochemical parameters were calculated.The values of association constant of the affinity reaction between 3-aminophenylboronic acid (APBA) and three mono-saccharides (glucose, fructose and galactose) were determined 1. Some physicochemical parameters such as enthalpy, entropy and Gibbs free energy of the reaction of 3-aminophenylboronic acid and glucose were also calculated assuming the formation of a 1:1 complex.2. A new method to detect hemoglobin with fluorescence Spectrometry was developed based on the quenching effect of hemoglobin for fluorescence of APBA. A linear relationship was found between the relative fluorescence intensity and the concentration of hemoglobin over 4.0×10^-7 to 3.0×10^-6 mol/L.3. A three-component system was established to detect the concentration of glucose in blood directly. Alizarin red S can combine with 3-aminophenylboronic acid to form five-membered rings. The addition of glucose made the scattering intensity enhanced. According to the increment of scattering intensity, we can detect the concentration of glucose. A linear relationship was found between the relative resonance Rayleigh scattering and the concentration of glucose over 2.0×10^-3 mol/L to 1.2×10^-2 mol/L and the detection limit for glucose was 1.8×10^-4 mol/L. The concentration of glucose in blood is at the range of 3.9×10^-3mol/L to 6.1×10^-3 mol/L. this method can be applied to the detect determination of glucose in blood.