Ecological Study On Functional Groups Of Microorganisms In The Water Body Of Internal River, Zhenjiang And Its Applications

Microorganisms play significant role in the energy flow and nutriments recycle in aquatic ecosystem. To study the distribution of physiological groups of bacteria and analyze the correlation between which with the main environment factors can accurately understand the status and role of bacteria in the aquatic ecosystem of Yangtse River.The subject is a crosshead of the function of biology on the control of pollution, that is the sub-issue of environmental improvement,repairing technique and synthetically demonstrating in Zhenjiang water environment. We make a survey of the population distribution of microorganisms in the water body of Yangse River and a study of microorganisms decontaminating organic contamination. These thorough literature,theory analysis and experiment is provided for the zoology repairing technique and supervision. The results show that:1．The distribution of functional groups of microorganisims in the water body of Yangse River were studied. The results showed that The the population of diversified microorganisims from internal river (4#,5#),Beigushan wetland (3#,6#) to the bank (1#,2#) increase and correlates with the content of correlated nutrient salt in water body. The correlation between heterotrophic bacteria and BOD₅ was 0．952 which reflect the water pollution level and be important indicators of water quality. The correlation between organic phosphorus decomposing bacteria, inorganic phosphorus decomposing bacteria and TP was 0．893, 0．865, respectively. The correlations between ammonifying bacteria and TN, NH₃-N were0．921, 0．899, The correlation between nitrifying bacteria and NH₃-N was 0．861．2．Organic phosphorus decomposing bacteria in the samling water of 1# spot is made bionomics observing and Physiology Biochemistry study on Jan ,2006 in order to providing evidence for identification. Molecular biology identification shows that this bacteria and Acinetobater junii have high homology. l6SrDNA sequence comparability and Molecular Phylogeny was performed based on analysis 16SrDNA sequence of organic phosphorus decomposing bacteria JD1．The result shows that organic phosphorus decomposing bacteria JD1 and some bacteria in Pseudomonas performed phylogenesis of decomposing dimethoate. Tolerant limit to pH severally is 5．0～5．5 and 9．0～9．5．The optimal pH is 7．0～7．5．The optimal time is 30～35℃and be about 32℃when bacterium mass reach the best high.3．Acinetobater junii could resist to dimethoate up to 500mg/L. bacterium growth isn't restrained .The optimal condition for growth and biodegradation were pH 7．2, temperature 32℃, liquid quantity 1/5, the quantum of inoculation 7%. Different original concentration influence the degradation rate for dimethoate so that waste water pretreatment should be considered in industry application.