Preparation And Chararcterization Of β-TCP/PLLA Hybrid Membrane Via Eletrospinning

The Tissue engineering of three-dimensional scaffolds were fabricated via electrospinning can support cellular in-growth and proliferation, because they have some characters with higher porosity, pore size adjustable and good biocompatibility allowing for cell migration. So the structure is help for the cell interconnect and infiltration and promoting the cell growth. In this work, we utilizes electrospinning with polymer solutions to fabricate new type ofβ-TCP/PLLA scaffolds with nanostructure fibers, then test and character the structure and performance of the scaffold, and we study the Cell Activity by in vitro degradation.Firstly,β-tricalcium phosphate nano-particles were prepared by an aqueous precipitation method from the addition of a diammonium hydrogen phosphate solution ((NH₄) ₂HPO₄) into a reactor containing a Calcium nitrate solution (Ca (NO₃) ₂·4H₂O), the structural properties and morphologies ofβ-TCP nanoparticles the powders were investigated by FT-IR, XRD, TEM and XPS. The results showed that the diameters ofβ-TCP particles are range from 219 to 328nm and with good crystal structure. Then we should optimization technics of electrospinning, we study the effects of process parameters on the fiber morphology, such as spinning solution concentration, voltage, feed rate and distance between the tip and receiver, by SEM and diameter distributing. At last the diameter of PLLA/β-TCP nanofíber range from 500 to 700nm can be produced via electrospinng under the optimization condition. The EDX was used to character the dispersion situation of PLLA/β-TCP in the hybrid membrane. Then FIIR, XRD and DSC were used to analyze the interfacial behavior. The surface bonding of PLLA/β-TCP is good forβ-TCP maybe react with functional groups of PLLA.Then we discuss the mechanical performance of PLLA and PLLA/β-TCP non-woven by mechanical testing. It was found that PLLA/β-TCP membrane has a strong surface bonding and high tensile strength of hybrid membrane due toβ-TCP. In wet conditions, the mechanical property of PLLA nonwoven has increased, but the mechanical property of PLLA/β-TCP nonwoven showed a downward trend.In vitro simulation is a usual method to evaluate bioactive performances of biomaterials. The main goal of this study is to attain in vitro degradation properties of composite scaffolds for the forthcoming animal study. Therefore, the remain part of this work is to study the influence of PBS solution (pH7．4, 37℃) over the degradation behaviors of scaffolds for up to 167 days. The performance of PLLA and PLLA/β-TCP non-woven were different. The pH of PLLA/β-TCP non woven was descending to a level and then take on some ascend trend due to import alkalineβ-TCP. The water take-up pace of PLLA/β-TCP is slower than PLLA woven, but the PLLA/β-TCP pace of mass loss and molecular weight was increase faster.At last, we analyze the Cell Activity by make the test of cell culture, it was found that the fiber is make for the cell adhesion and reproduce go with importing theβ-TCP to make the diameter and coarseness increased.