| The reduce of finite reserved fossil fuel and the continual increase on energy sources requirement and environment pollution resulted in fossil fuel burning, 21th century energy will be confronted with great challenge. So reproducible and clean energy sources will be the principal part of the continuable development energy sources system in the future. Hydrogen is clean and renewable. It only liberates large amount of energy and water after combustion, does not evolves the greenhouse gas. Technology with microbe for hydrogen production needs reaction condition geniality and the wastage of the reaction system is low. So it gives a solution for the conflict of energy and environment. since the technique of cell immobilization has many advantages, it has been far and widely used in many fields,such as industry, physical, chemistry analysis, environment protection and so on At a certain extent, it can increase microbe quantity in reactor by the combinantion of technique of cell immobilization and technique with microbe for hydrogen production together. As a result, the rate of hydrogen production and the stability of circulation could be improved. This article used a sort of high efficiency Photosynthetic bacteria (PSB) which was cultivated and filtered by ourselves, investigated the characteristic of hydrogen production and glucose degradation for a single immobilized cells granule. And set up the elementary academic model of internal mass transfer profiles including reaction in immobilized cells granule. The main contents and parts of conclusion were listed as follow:①This article discussed the factors which effect the characteristic of immobilized cells granule. These factors mostly include carrier concentration, ratio of mixed carrier, temperature, delay time, impregnant concentration and so on.②According as PSB particularity, this paper prepared different kinds of immobilized gel sphere by varying some technical factors, such as BiH3O2 concentration, reaction temperature, ratio of mixed carrier, and so on. Those particularity parameter of gel granule obtained were satisfying In the experiment, efficient diffusion coefficient, porosity factor, stability, intension etc for all kinds of immobilized granule were measured. And it was observed that the gel sphere interior structure, analyzed the change of interior structure after cultivated in different period..③Designed subminiature bio-reactor and built experimentation system for hydrogen production and glucose degradation by immobilized cells. The bio-reactor should satisfy that it is feasible to observe the microcosmic reaction phenomena in the degradation reaction progress. In this part, it could be observed that the reaction process in series and the form of outcome was observed principally. The result indicated that the transmission course of produce was accomplished by the way of molecule diffusion. And there was no bubble in immobilized cells sphere.④Concerned to the hydrogen production kinetics for immobilized PSB cells, this paper investigated those about the effect of substrate concentration, light intensity, temperature, substrate pH, velocity of substrate flow etc on the glucose degradation and hydrogen production for immobilized cells. Experiment obtained the regulation of all factor concerns about the glucose degradation and hydrogen production. it was also indicated that optimum reaction condition were determined as initial pH 7, temperature 30℃, light intensity 6000lx, substrate concentration 60mmol/L, velocity of flow about 22ml/h, adopting yellow light as incidence light. There were large pertinence between the rate of hydrogen production and glucose degradation. A small warp consisted in the effect of substrate concentration.⑤Elementary academic model of internal mass transfer profiles was set up and the bio-reaction in immobilized cells granule which combines the effect of light intensity, temperature and substrate pH. were take into account The calculation results and the experiment results were compared and analysised in this work.
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