| Along with the living level raising continuously, people are more and more concerned over the health of themselves.Especially the safe of agricultural product is spoken of very high concern level.But, in recent years, it is not very fresh to see poisoning affairs because of polluted food, polluted water taken or miss taken pesticide.In this study, high performance liquid chromatography instrument is used to detect pesticide residue in biological samples . The thesis mainly includes following contents:Investigations had been performed on the determination of paraquat. After many times experimentation, developed a new fast method to analyze paraquat in urine by Spectrophotometry. Paraquat at a concentration range of 0.5-10μg/mL in urine were determined with high accuracy and precision The relationship of the peak current and the paraquart was y=0.01548+0.22109x, (r=0.99985). In study of the effect of the pre-treated method, the mobile phase, ion-pair reagent, the pH, and other additive stauff .At last we chose water and acetonitrile at 9: 1 as the mobile phase, added 1-Heptanesulfonic acid (5mmol·L-1) and triethylamine(40mmol·L-1). pH was adjusted to 3. The relationship of the peak current and the paraquat concentration was in the range of 100ng·mL-1—500μg·mL-1 (r=0.99974). The detection limits of paraquat was 25ng·mL-1, the recovery was 88.28%-95.47%. The method had been applied to the determination of paraquat in urine satisfactory result.Investigations had been performed on the pre-treated method , the mobile phase, , the pH, the temperature and other stauff. The urine was pre-treaed by acetonitrile.The mobile phase was water and methanol at 1:1 The flow rate was 1.0mL/min, and the temperature at 35℃. The UV-VIS detector wavelengh was adjusted to 280nm. The relationship of the peak current and the paraquat concentration was in the range of 0.2μg/mL—10μg/mL(r=0.999l5). This metod was very economy, it could be used to inspect the concentration of carbofuran in urine.
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