Studies Of Isolation, The Phenol-degrading Gene Locating And E.coli Expression Of A Phenol-degrading Bacterium

The phenol-degrading bacteria was isolated from coking wastewater treatment plant's Activated Sludge.The strain with highest phenol-degradating ability was choosed for gene orientation and extracted its degradation plasmid into E.coli to express.Achieved better results.The Specific findings are as follows:1.Five effective strains were isolated from the activated sludge and identified the best phenol-degrading bacteria H for the study by comparing the five bacteria phenoldegrading effect through 4-Amino-4-direct spectrophotometric analysis.2.Three were extracted from from the high phenol-degrading bacteria strains H The largest plasmid was more than 10 kb and the smallest about 2 kb.3.Phenol-degradating gene orientation analysis to H:plasmids of H were eliminated through SDS and protoplast regeneration respectively and The results showed that capacity of H disappeared with removal of plasmid,so phenol-degradating gene was mapping in the plasmid preliminaryly.4.The plasmid extracted from H transformed into E.coli to express and obtained transformants.5.The results of Phenol-degradating capacity test results showed that transformants got better results Phenol degradation ratio was 77.34%in 12h,but still was than H, which may be related to copies of the plasmidthe transformants contained.And it also proved transformants obtained the plasmid with the same characteristics as H.E. coli expression system with phenol-degradating capacity layed a good foundation for the application of biotechnology of degradation of pollutants,witch had great theoretical and practical significance.