Study On Extraction,Analyses And Bacteriostatic Action Of Aroma Composition Of Zanthoxulum Spp.

The material of this experiment is Zanthoxulum spp.from Hongya and Jinyang of Sichuan.The experiment mainly filters the extracting methods of the aroma composition of Zanthoxulum spp.,then extracts the aroma composition of peek seeds and leaves of two kinds of Zanthoxulum spp. from Hongya and Jinyang of.At last,researches the bacteriostatic action of the peels aroma composition extracting by different methods.The experiment provides bases for deep processing and overall utilization of Zanthoxulum spp..1.Using distillation,solvent extraction and SCFE to extract the aroma composition of Zanthoxulum spp., the experiment gropes and optimizes the technology parameter of the three methods, then analyses the aroma composition extracted by different methods by gas chromatography-mas spectrometry.The result indicates:The loss of evaporability composition is least when the material dried 3 hours under 70℃.The yield of distillation is lowest(8.42 ml/100g),but the cost is lowest too.Selecting aether as the solvent,the infection order of every factor of solvent extraction to the extracting rate is: waterbath temperature＞time＞ratio of dosage liquor.The best extracting condition is:grade size 20-40 screen mesh, waterbath temperature 40℃,time 2h, ratio of dosage liquor 1:30,the yield is 9.81%.Because the solvent can be used circularly,the cost of this method is lower and the yield is higher.Because of the remanet of solvent,this method can be used in the industrialization production except food industry.The infection order of every factor of SCFE to the extracting rate is: temperature＞pressure＞time＞fluence.The best extracting condition of SCFE is:temperature 55℃＞pressure 40 MPa,time 2.5h,fluence of CO₂ 20 kg/h, the academic yield is 11.2% and the actual yield is 10.89%.When putting in ethanol that the volume is 3% of the extacting kettle and concentration 95%,the yield reaches 13.2%.The cost of this method is highest but it is convenient and efficient,and the yield is highest.And there isn't remanet of solvent in this method.2. Uses GC-MS to identify the aroma compositions extracting by different methods.There are 24 kinds of compositions in distillation,mainly the fragrance element including Linalool,Limonene,cis-beta-Ocimene,beta-Phellandrene,4-Terpineol and so on; there are 39 kinds of compositions in solvent extraction, including Linalool,Limonene,beta-Phellandrene,Germacrene,Myrtenol and so on; there are 54 kinds of compositions in SCFE.Excepting the kinds previously tells, there are Undecylenic acid,Dibutyl phthalate,Z-11-Hexadecenoic acid and so on.Acording to c the sort of the aroma substance,SCFE is the best method.3.Using GC-MS,the paper analyseses and contrasts the products extracted from different parts(peel,seeds,leaves) of the two kinds of Zanthoxulum spp.extracting by aether. The result indicates:every parts of the Zanthoxulum spp. of Jinyang contain more aroma compositions than the breed of Hongya. The two kinds of peels contain 21 kinds of identical composition such as LinalooK Myrtenol,Germacrene and so on,which account 78% and 74.87% of the total detection; The two kinds of seeds contain 12 kinds of identical composition such as Hexadecanoic acid, methyl ester,9,12-Octadecadienoic acids Myristicid and so on, which account 94.5% and 94.22% of the total detection; There're 30 kinds of identical composition in the two kinds of leaves,but the ratio of them is lower,it is 69.86% and 46.52%. Content of Limonene is high in every part of Zanthoxulum spp. of Hongya while it is low in the breed of Jinyang.4. Aroma composition extracted by different methods have distinct extent bacteriostatic action to Escherichia coli,Staphylococcus aureus,Candida tropicalis,Candida utilis,Geotrichum candidum,A. fumigatus and A spergillus niger,but have no action to Trichoderma varide Pers.ex Fr.The depressant effect of the aroma composition extracted by distillation is best,and the other two are alike.The bacteriostatic effect of Hongya Zanthoxulum spp. on Candida tropicalis,Candida utilis and A. fumigatus is better than that of Jinyang,but infirmer on Escherichia col,Staphylococcus aureus,Geotrichum candidum,A spergillus niger.