| In our country, the poultry blood is produced the most than other countries,which reach 1.9×109kg every year, contain protein 3.6×108kg. Because the rawmaterial blood is extremely difficult to preserve, and extremely easily polluted. Thesmell, digestion and the sense organ factors have seriously restricted the poultry bloodcomprehensive utilization, and caused the massive precious poultry blood resources towaste, and polluted the environment. The poultry blood is decomposed bymicroorganism, and the method is recognized as a blood comprehensive utilization,because the invests and the energy consumption is less than others, but the protein,peptide and amino acid content are more than others,This thesis isolates and screens 16 kinds of bacterial strain from the soil. Astrain,(code name:Lact5.Ⅲ),is determined as the study object final, according to it'sprotein hydrolysis ring ratio, protease activity and rate of change of the free aminoacid, soluble protein etc. while the pure blood hemoglobin is fermented, and someproducing protease peculiarities of the strain (code name: Lact5.Ⅲ) are analyzed anddeterimined. The original bacterial strain(code number: Lact5.Ⅲ) and mutation strainbelong to Bacillus ofB.cereus, which is identificted by way of bacterial strain colony,shape and physiological biochemistry test, and classify retrievement table of thebacterial strain.Fermentation in the liquid of the animal hemoglobin (Hb) by Bacillus ofB.cereus,The result shows: this bacterial strain is suitable to grow in the acidenvironment (pH6.5) among 30～40℃of fermention temperature; Ca2+and Spain 20can promote to produce protease.Carry on research on Hb decomposing mechanism,The result shows: Leadedorientation in the hemoglobin (Hb), This bacterial strain's protease activity impovesfrom 483.81U/ml to 731.22U/ml; The decomposing rate of lib rises from 40.25％to65. 84％. Content of soluble protein and amino acid increase from 2.69％, 1.44 mg/100ml to 4.12％, 2.56 mg/100ml respectively. After this bacterial strain is mutatedorientation, protease activity is strengthened to rise from 483.81U/ml to1233.15U/mlof the fifth generation mutation bacterial strain, Content of soluble protein, aminoacid and decomposing rate of Hb increase from 2.69％, 1.44mg/100ml, 40.25％to3.02％, 2.41 mg/100ml, 51.83％separately.The results analysis of decomposing the animal hemoglobin (Hb). Flattypewriter ribbon of original bacterial strain (No1) is wider than mutation bacterialstrain (No2) in standard kind horizontal line (M) and the number of typewriterribbon's mutation bacterial strain (No2) is more an obvious than original bacterialstrain (No1) from SDS-PAGE gel electrophoresis picture. Determine and analyse ofthe result's content of amino acid, The total content amino acid of mutation bacterialstrain is more 5.75％than original bacterial strain bacterial strains. Phe, His, Met etc.raise by more 90％.but Thr, Glu, Pro, Arg drop to-360％,-1.08％, -340％and -485.71％separately; Except Thr, Cys, Phe, His, Arg decreasing slightly, the other 13kinds of amino acid and these total amount increase obviously than original bacterialstrain.Application of the fermented blood. Fermented blood product is produced byadding fresh blood mixture as fermented raw materials, such as maize powder,powder of dregs of beans, etc. by way of adopting many bacterial strains solid state toferment effectually. The product has some characteristics which the content of aminoacid is 100mg/100g, which the soluble content of protein is up to 20％, which theproducts are pale red, the sweet-smelling is strongly, have fragrance with specialfermented blood, there is no peculiar smell.
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