Studies On Extraction And Enrichment Technology Of Flavoniods From Celery

In this paper, flavoniods content of different celerys of Hunan province weremeasured by spectro photometry, the processing conditions of leaching flavoniodsfrom celery were optimized with orthogonal design, and flavoniods were enriched bymacro-porous adsorption resin. Then flavoniods were detected by color reaction, TLC,HPLC and UV spectrum. The detailed experimental results were as follows :Firstly, using spectro photometry plotted the standard curve of flavones. Theregression equation was y=0.9967x+0.0178, R~2=0.992. Based on this method, wecompared flavoniods content of celery leaves and celery stems which we collectedfrom different areaes of Hunan province. The results showed that flavoniods contentof Zhangjiajie wild celery was the highest, and flavoniods content of Shaoyang celerywas the lowest .The highest was about fourteen times higher than the lowest. Theflavoniods content of celery leaves were about quadplex higher than stems.Secondly, through comparing methanol, alcohol, ethyl acetate, n-Butanol,chloroform in extracting flavoniods from celery, we knew that alcohol was theoptimal extraction solvent. The optimum alcohol soaking technology parameters hadbeen obtained as follows: the concentration of alcohol was 70%(v/v), the ratio ofmaterial and alcohol was 1:5(w/v), the time of extraction was 30 min and thetemperature was 80℃, extractced twice under the technology parameters. Theoptimum alcohol ultrasound technology parameters had been obtained as follows: theconcentration of alcohol was 50%(v/v), the ratio of material and alcohol was 1:25(w/v),and the time of extraction was 9min, extractced twice under the technologyparameters.Thirdly, Six types of macro-porous adsorption resin were selected to compare theirperformances in absorbing and desorbing celery flavoniods. Experimental resultsshowed that XDA-1 resin was optimal choice for flavoniods from celery, meanwhileoptimal adsorpt and desorpt technology parameters were obtained: the currentvelocity was 2BV/h, the eluting solvent was 70% alcohol, the flow rate of elutingsolvent was 2BV/h, the volume of eluting solvent was 4BV. The purity of flavoniodsin the product was 10.52% when the flavoniods content of celery extraction was1.25%.At last, color reaction, TLC, HPLC and UV spectrum were used for analyzing thecomposition of celery flavoniods. Results indicated that apigenin was predominant omposition in celery flavoniods. Based on the mobile phase chloroform- methanol-water(18:2.3:0.35), the high performance thin-layer chromatographic method fordetermining apigenin in celery flavoniods was established. The regression equationwas y=15138x+7594.8, R~2=0.993, and the linear relationship was at the rang from0.332ug/spot to 0.996ug/spot. The content of apigenin in product enriched by theresin was 48.6 mg/g.