Study On The Microbial Degradation Of Isoquinoline And 2-Metheylquinoline

Regard two heterocyclic compounds isoquinoline and 2-methylquinoline as the researchobjectcs, batch experiments were carried to study the degradation characteristics and focus on theoptimal C/N ratio, variation of nitrate, nitrite, and isoquinoline and 2-methyquinoline and so on.Meanwhile, the pathways of anoxic and anaerobic degradations were analyzed.The following conclusions could be summarized from the experiments: (1) Onmono-metabolism, the degradations of isoquinoline and 2-methylquinoline under anoxicconditions were better than under aerobic and anaerobic conditions.. (2) On mono-metabolism,the degradations of isoquinoline and 2-methylquinoline (20～100mg/L) under the three conditionconformed to zero-order reaction kinetics. And the degradation rate of isoquinoline is lower thanof 2-methylquinoline. (3) Under anoxic condition, the optimal C/N ratio of isoquinoline and2-methyquinoline respectively were. 104～110 and 25～30. They are both less than the optimalC/N ratios of other heterocyclic compounds (i.e, pridine, indole and quinoline). (4) The changescopes of pH and ORP in anoxic and anaerobic conditions are small, so they couldn't be used todefine the proceeding of the degradation process. (5) During the degradations of isoquinolinewith or without nitrate, 1(2H)-Isoquinolinone was detected as the intermediate product, whichsuggested that the anoxic and anaerobic pathways was the same as the early pathway of aerobicdegradation and also proved the ubiquity of hydroxylation during the degradations ofnitrogenous heterocyclic compounds. (6)During the degradations of 2-methylquinoline with orwithout nitrate, 4-ethyl-Benzenamine was detected as the intermediate product, which suggestedthat the anaerobic pathways was the same as the early pathway of anoxic degradation. A moretoxic substance m-Chloroaniline was detected in mono-metabolism during the anoxic condition.