Isolation, Identification And Characterization Of Atrazine-degrading Bacterium ADX10

A bacterium strain named ADX10 was isolated from atrazine-contaminated soil, which was capable of utilizing atrazine as the sole carbon and nitrogen source for its growth. This bacterium was identified as Arthrobacter sp. based on its physiological and biochemical analyse and 16S rDNA series same source analysis(GenBank Accession No. EF488200).Biological properties of strain ADX10 was studied. The optimal pH for its growth was 6.0-9.0, the optimal temperature was 25-37℃, and the optimal osmotic pressure was 0-3g NaCl/L. All the monosaccharose in the test can be well used, except sorbitol and starch. All the organic and inorganic nitrogen source can be used well.The optimal pH for degrading atrazine by strain ADX10 was pH6-10, optimal temperature was 20-35℃. The strain ADX10 can degrading 500mg/L atrazine in 48h by HPLC test.Got the degradation genes via PCR, all the three genes have 99% similarity to the relevant genes in the strain ADP and TC1,. We have found Cl~- in the degradation product liquid and cloned the gene trzN which can release Cl~- from atrazine, and got the gene atzB, atzC, we concluded the reaction product at least to Cyanuric acid based on these data.Got the whole gene trzN, and found it had two mutations from the reported one, and the peptide chain had two mutations too, but the strain had no difference on degradation from the strain TC1.