The Study Of Detecting Inethod For Clenbuteroli Hydrochloridum During Slaughtering Of Pigs

Clenbuteroli hydrochloridum is a beta Adrenalin. Long-term or go beyond standard abuse it, not only reduces the quality of animal products, but also endangers human health. A variety of Clenbuterol detecting methods have been established domestically and abroad. The detecting methods currently used mainly enzyme immunoassay analysis, HPLC and GCMS analysis. For complex biological system, the enzyme immunoassay method has false positive phenomena. The HPLC sensitivity is low and easy to be interfered by other components of the sample. GC-MS has high sensitivity, but the samples need to be derived, so the sample pre-treatment is complex. Therefore, detecting method studies for the clenbuterol during the slaughtering and processing of pig are necessary.In this paper, several different methods for Clenbuterol extracting from the samples were compared. Finally, acetonitrile - hydrochloric acid solution extracting method was identified. In this method n-hexane is used to remove fat and fat-soluble impurities, and chloroform is used to remove water. After the solution was concentrated to remove chloroform, the dry Clenbutrol was diluted with methanol and then inspected with machine.This pre-treatment process is simple, at the same time, has relatively high recovery rate. So it meets the requirements of the determination of Clenbuterol.High performance liquid chromatography and liquid chromatography - MS determination of clenbuterol were compared too. HPLC has a low sensitive detection limit of 20μg/kg. LC-MS has a high sensitive detection limit of 2μg/kg and a high separation efficiency which can greatly improve the monitoring of the stability, accuracy, reproducibility and the detection limit. The parameters of the Chromatography and Mass Spectrometry conditions are optimized. An accurate and rapid detection method for detecting Clenbuterol has been established in this paper.The detection time, cost, the detection limit and equipment characteristics between HPLC, LCMS, ELISA kits and rapid cards detection were compared. Rapid cards detection is fast, but the detection limit is relatively high. And the false positive rate was higher. So it suit for pre-slaughter detection for large quantities of live pigs. ELISA kits can detect more than 40 samples one time. Testing time is three hours. Cost is low. But it has false positive phenomenon too. It suit for pre-slaughter urine filtration of pigs. As for the after slaughter tissue samples testing, LCMS is a corroborative method for identifying the quality and quantity of Clenbuterol due to its accuracy and rapid speed.