Optinization Of The Extraction And Purification Technology Of Gentiopicroside From Gentiana Macrophylla Pall

Extraction,separation,purification of gentiopicroside from Gentiana macrop-Hylla Pall, produced in GanSu have been studied in this paper..The qualitative and quantitative analysis method was established by HPLC,UV with normal GPS as comparison. The method can offer favorable evidence to analyze GPS in the course of Extraction and separation.The experiments were in accordance with the national standard GB/T5009.3-2003,GB/T5009.6-2003,GB/T5009.4-2003,GB/T5009.10-2003 in measuring gentiana original herb moisture,fat,ash,crude fiber content, the results showed the average levels for targets above as followed: 11.41 %, 9.284%, 4.094%, 12.34%. Parallel experiments in various deviations were small, the measurement results were relatively credible, this can regard as right medicine general chemical composition of gentiana.On the basis of the single factor, using gentiopicroside as the index, the effects of ethanol amount, extraction time, extraction degree and extraction temperature on extraction technology were evaluated by orthogonal design method and UV. The superior technology of gentiopicroside was as followed: the ethanol was 30 ml; extraction time of 20 min, the number of extraction 4th, and the temperature was 50℃. The effects of water amount, extraction time, and extraction degree and extraction temperature on extraction technology were evaluated by orthogonal design method and Ultrasonic Wave. The superior technology of gentiopicroside was as followed: the ethanol was 15 ml; extraction time of 40 min, the number of extraction 2nd, and the temperature was 40℃.Optimized Soxhlet ethanol method, using gentiopicroside as the index, the optimum condition is the solvent dosage of 180 ml; extraction time for 2.5 h.Using cold-immersion method, by orthogonal design the optimal extraction condition was confirmed. The superior technology of gentiopicroside was as followed: ethanol concentration of 60%, extraction time of 12h and feed ratio of 1:9, 2 extraction times. Choose 8 types of resin for gentiana water extract of gentiopicroside adsorption -desorption experiments, the results showed D-101 resin adsorption - desorption method was better than others. D-101 resin selected adsorption - desorption conditions were as followed: pH within 2 -3, salt concentration of about 1.0 mol / L.