Study On Preparation Of Haemoglobin Polypeptide-iron And Its Anti-anaemia Function

There are abundant proteins and amino acid in the porcine blood and its nutrient value is high. Haemoglobin is an important ingredient in the porcine blood,which is more than 75%of total protein in the porcine blood and more than 90%of total blood corpuscle protein.Iron deficient anemia is one of the most common anemias in the world,and is also an important global nutrition deficient disease.The polypeptide-iron is a novel bio-active iron with stimulativeless and high bioavailability,which can be absorbed directly by the intestinal cells.Therefore,haemoglobin polypeptide is gotten from fresh porcine blood;then,based on the chelating response between molysite and haemoglobin polypeptide,polypeptide-iron can be processed,and its anti-anemia results were aslo studied in this paper.The results showed as follows:1.The fresh porcine blood is used as material in the research.In the preparation process of haemoglobin polypeptide,the conditions of the discolouration,desalination and ultrafiltration and its sensory evaluate were discussed.The results indicated that:(1)The optimum parameters of the discolouration process are activated carbon concentration 2.0%,pH 5.0,temperature 50℃and time 1.5h.(2)The best flow velocity of the desalination process is 2BV/h.(3)The optimum parameters of the ultrafiltration are the operation pressure 0.15Mpa and temperature 25℃.(4)The haemoglobin polypeptides are divided into four kinds of the molecular weight scopes by ultrafiltration.The molecular weight scopes are more than 10kDal,5-10kDal,1-5kDal and less than 1kDal,and the content is 4.38%,13.97%,74.76%and 6.89%respectively,the most of polypeptides is 1-5kDal.(5)The haemoglobin polypeptides are canary powder,slightly bitter and without blood odor.2.The synthesized conditions of haemoglobin polypeptide and ferrous chloride were studied, and the component of the product has also been discussed.The results indicated that:The best chelating process conditions is the ferrous chloride chosen for the iron source of chelation,pH 5.0, the ratio ofpolypeptide to ferric chloride 3:1 and 3.0%polypeptide concentration.It is proved that the polypeptide-iron is a new product in the form of chelation and is different from the haemoglobin polypeptide and the ferrous chloride.3.By the means of low-iron diet and weekly tail vein bleeding,the iron deficiency anemia rat model was successful accomplished after four weeks.And it is proved that the polypeptide-iron can improve the effect of the iron deficiency anemia in rats,and anti-anemia results are clearly better than the ferrous glucose acid and ferrous chloride.Therefore,The polypeptide-iron can be used as an efficacious iron preparations for improving the iron deficiency nutrition anemia.