Analysis Method Of Mercury And Methylmercury In The Habitats Of Rana Chensinensis

This paper replied on the National Natural Science Foundation (40673059) to start the experiment. In this paper, concentrations of total mercury and methylmercury were determined in Rana Chensinensis, such as skin, muscle, offal, oil Rana (female), and eggs (female). The concentrations of total mercury and methylmercury were also determined in soils, sediments, and water where Rana Chensinensis habitats live. The conclusions were listed as following:1. The correlation coefficient is over 0.99 by using AFS (Atomic Fluorescence Spectrometry) to measure the total mercury in 30 days. The result shows that the instrument has a good stability.2. The detection limit of AFS is 0.992μg·L^-1 in this paper.3. Wet digestion was used to deal with water samples and soil samples, the average recoveries of total mercury are more than 80 percent. So the method is applicable for the pre-treatment of the total mercury in water samples and soil samples (including sediment samples).4. Wet digestion and microwave digestion was used to deal with Rana Chensinensis samples. The average recoveries of total mercury are more than 80 percent. So the two methods are applicable for the pre-treatment of the total mercury in Rana Chensinensis samples.5. Pre-treatment approach selected should be depended on the actual samples. When the Rana Chensinensis samples are less and there are enough dissolving cups, it is better to use the method of microwave digestion. When the biological samples are larger, the wet digestion should be considered for the pre-treatment. However, cross-contamination may happen in this method.6. The detection limit of GC is 0. 0246mg·L^-1 in this paper.7. The retention time of 1mg·L^-1 methylmercury is stable in near future, which would shows stepped changes in long time. It is the best that the determination of same samples is completed in 10 days.8. The fluctuation of the peak area of 1mg·L^-1 methylmercury is large in 60 days. In the process of the determinations of actual samples, the standard curve must be determined firstly in order to ensure the accuracy.9 Samples in various parts of Rana Chensinensis and the environmental samples where they live can be determined by using the national standard methods of pre-treatment.10. Capillary column in GC (Gas Chromatography) can be used for the determination of methylmercury analysis in environmental samples of Habitat Rana Chensinensis. Because of the characteristics of methylmercury and complexity of environmental samples, it still need further study for using of capillary column to continuously analyze a large number of samples.