Biodegradation Of 2, 4-Dichlorophenol From Aqueous Solution By Phanerochaete Chrysosporium

In order to remove the CPs' pollution soil or wastewater,we adopt several techniques to bioremedy the environment.Compared with the white rot fungi technology,the traditional physical and chemical method are more expensive,inefficiency and often cause secondary pollution. Similarly,some biological methods are intolerable to the high concentration pollution and can not biodegrade pollutions completely. However,the white rot fungi technology,which attracted more and more attentions,could avoid these limitation easily.Thus,it is necessary to develop a new technology to deal with CPs' contamination and research the white rot fungus' biodegrade mechanism.In this article,the biodegrading effect of 2,4-DCP from aqueous solution by Phanerochaete chrysosporium was studied.We established the main optimum conditions of the enzymes activity of LiP and MnP on the Phanerochaete chrysosporium,such as immobilized spore dose,pH, rotating speed and temperature.The CPs-pollution water was treate at the optimum conditions by using immobilized Phanerochaete chrysosporium. The factors of initial 2,4-DCP concentration,surfactant concentration and the metal cation were investigated througly. The results showed clearly that the stability of enzyme production and activity were remarkable improved after immobilization of the free Phanerochaete chrysosporium.After inoculated immobilized spore dose 10 mL(spore dose 1×10⁵ spore/mL),pH 4.5,rotating speed 180 r/min, temperature 38℃,the activity of LiP and MnP enzyme reached to 56.3 U/L and 84.1 U/L.In the existence of 20mg/L 2,4-DCP,the biodegrading effiency could reach to 55%after 12d.1%w/v surfactant Tween-80 had been showed to enhance the bioremediation ability.The biodegradingn activity is also inhibited by Fe²⁺and 10mg/L Cu²⁺,Mn²⁺and 1mg/L Cu²⁺can improve the removal efficency of 2,4-DCE...