| With the development of nano technology, two or more kinds of nano-materials synthesized each other has been extensively studied in recent years. The magnetic nano-particles packed with SiO2 has also been concerned since their significant functions and applications in biological medicine, cell technology and biological engineering. Their advantages include good biologic compatibility, low biological activities which can change the diffusion rate and their functions. The hydroxyl function group of these particles makes it bond with the target molecules and react with ethanol and silane coupling agent. After the absorption on to the magnetic particles, proteins and cells can be separated and purified with the help of magnetic fields.Through the process of packing the magnetic particles with fluorescence materials and SiO2, the magnetic fluorescence nano- particles can be obtained. These nano-particles show great applicability in the fields of cell separation, immunity examination, cell imaging, target medication track, and so on.The thesis is mainly studying the preparation and application of the immune magnetic nano-particles which were prepared by using the chemical co-precipitation and then packed with SiO2. The particles can be diffused well in solution. And then, the nano-particles were modified by chemical reagent. The nano-particles can be used for separating the cells. The large increment of cells indicate that the whole process works very well including the preparation of magnetic nano-particles in the aqueous solution which avoids any possible contamination. In this study, some results have been obtained and shown as follows:(1) The superparamagnetic nano-particles were synthesized using the chemical coprecipitation method.Fe3O4 nano-particles had good dispersion, high purity and spinel crystal form. The average diameter of the particles was about 30nm, and the saturation magnetization intensity was 73.1emu/g. The biological compatible magnetic nano-particles were obtained by coating silica onto the surface of nano-particles. The bio-functional magnetic nano-particles were then modified by amino group and monoclone antibody. Finally, the immunity magnetic nano-particles were obtained. Using these particles, cell separation of the product and the purification can be carried out in a magnetic field.(2)When mixing phen-benzoic acid rare-earth composition into Fe3O4, it showed that the structure of the composition was not destroyed, so the immune magnetic nano-particles had good fluorescence. In the main fluorescence emission spectrum of the magnetic fluorescence nano- particles, the fluorescence intensity was about 441.7.(3) The target cell could be separated rapidly by magnetic nano-particles which had no influence on the activity and the shape of the cells. Comparing to other separation techniques, this method had many advantages such as, simple, rapid, accurate in operation, and so on. This method can separate highly purified CD133 from the cord blood within a short time.(4) With the usage of, for the first time, self-made immunity magnetic nano-particles, the CD133 cells were separated and purified from the navel blood. The red, granule colony was formed after the single nuclear cells (comparison group) and the CD133 cells (experimental group) were cultured for 14 days and 21 days respectively. The culturing results indicated that the CD133 cells, which were separated with self-made immunity magnetic nano-particles, had good activities and increment, formed the red granule colony. The multiplying increments of the red, granule ancestor cells colony, which were obtained by culturing the single nuclear cells(comparison group) and the CD133 cells(experimental group) for 14, 21days respectively, were compared. And the results indicated that CD133 cells, which was the symbol of haematopoietic stem cell, had stronger multiplication ability (P<0.01).So, this technique can be employed in the clinics and all kinds of blood diseases.
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