Biodegradation Of Cinnamaldehyde And Cinnamic Acid And Its Product Analysis

In this paper, it was studied the process of cinnamaldehyde and cinnamic acid biodegradation and the product analysis, it was established qualitative and quantitative analytical methods of a complex biological reaction system. On the basis of which it was reseached the conditions of screening and conversion with cinnamaldehyde and cinnamic acid as substrate, and the research results are as follows:(1) A simultaneous quantitative analytical method of cinnamaldehyde and cinnamic acid was set up by HPLC. The best chromatographic conditions were methanol-water-acetic acid (55:45:0.05) as the mobile phase, and the UV detection wavelength was 246nm. In the best possible conditions, we can got acetophenone, cinnamic acid linear equations are: Y=32017X-6298.6, Y=11286 X-4508.7.In addition, the method is validated by accuracy and recovery experiments.(2) The UV-absorption characteristics of cinnamaldehyde,benzaldehyde and benzoic acid were discussed, and the quantitative analysis method was developed, The results showed cinnamaldehyde and benzaldehyde had the same absorption in 228nm and 256nm. A simple and rapid method of quantitative analysis mixtures was established with the use of equal-absorption point. The relationships between concentration and absorbance were given. The relative standard deviation (RSD) of precision of mixture was <1%, the relative standard deviation (RSD) of its stability was <1% and the recovery for them was 99%-101%.(3) In a total of 122 soil samples collected in the peak forest, the peak GUI refineries, Yulin-South, experiment of the strains' separation and biodegradable strains' screen were taken, and selected 186 strains. In them, there were 6 strains which had the ability to transformation, one higher transformation ability strain (5^#) was taken to cinnamaldehyde's biodegradation experiment and the transformation conditions were optimized. And moreover, Fermentation conditions such as carbon, nitrogen, temperature, pH of initial fermentation and medium volume were optimized. The results showed that the best conversion conditions were glucose as the carbon source, beef extract as nitrogen source, at 30℃, and the initial pH of 5.0, 100 mL medium volume, the content of benzaldehyde was 5.24%.(4) High conversion strain was screened; it could biodegrade cinnamic acid into acetophenone effectively and selectively. By identified, the bacterium of the genus is Burkholderia Vietnamiensis bacteria.(5)The conversion conditions of Burkholderia Vietnamiensis bacteria transform cinnamic acid to form acetophenone had been optimized. When optimum conditions were sucrose as carbon source and concentration of 2g·L^-1, peptone as nitrogen source and concentration of 2.5g·L^-1, at 30℃, fermentation for 2.5 days, and conversion for 2 days, the transformation solution in the pH of 9.0, the conversion of 0.5g cinnamic acid substrate, the content of acetophenone was up to 3.24 g·L^-1, conversion rate of cinnamic acid was 97.2%.