| The Room Temperature phosphorescence is a analyze method with wide linear range and higher precision, lower limit of detection, higher selection. The method is operated much fast, more simply and fewer investment for general analyze.As a new focus in the field of optical chemical sensors, The original idea of liquid-drop chemical optrode is constructive in exploiting some unique features of liquid drop, namely, reproducibility, renewability, the lack of containment wall and lens like optical cell. The infection of optical signal caused by the absorption and scattering of cell surface or the contaminations of reagents and samples to cells in conventional photometric analysis can be avoided. The unique features of a liquid drop can be characterized in its reproducibility, renewability, defined volume, and lack of containment walls. The features individually or in combination, may result in high sensitivity and accuracy for analyses.In this paper, the technique of droplet optical chemical sensor has been applied in room temperature phosphorescence. A novel method of drop-based analysis, which regarding LS55 luminescence spectrometers as its experiment window, has been developed, many new phosphorescence chemical sensors combined with flow-injection analysis and the technique of dynamical drop-based sensing are developed. The apply domain and area of droplet optical chemical sensor has been extended.The contents of this thesis include as following:1. A room temperature phosphorescence based on renewable optical chemical biosensor combined with flow injection technique has been developed for the determination of o-phenanthroline.The RTP of 9-bromophenanthrene can be induced by sodium deoxycholate (NaDC) in the presence of oxygen, but it is obviously quenched by trace o-phenanthroline. A method of flow injection renewable drop (FIRD) phosphorescence for monitoring o-phenanthroline was established. The experimental conditions was investigated and discussed. Under the optimum conditions, the linear range was 4.0×10-7 mol·L-1to 4.0×10-5mol·L-1, and the limit of detection was 0.031μg/mL. This method has been applied to the determination of o-phenanthroline with satisfactory results.2. Iron ion can quench 9-chlorophenanthrene, 9-bromophenanthrene, 9-Iodophenanthrene RTP of reducing byβ-CD. Based on this reaction, the content of iron ion was determined utilizing renewable droplet optical- chemical phosphorescence sensor. Under the optimum conditions, the sensor shows linear response in the range from 4.0×10-7 mol·L-1-6.0×10-4 mol·L-1 in 9-chlorophenanthrene solution. The regression equation is I0/I-1=0.027+0.67×104C. The sensor shows linear response in the range from 4.0×10-7 mol·L-1-6.0×10-4 mol·L-1 in 9-bromophenanthrene solution. The regression equation is I0/I-1= I0/I-1=0.39+1.34×104C. The sensor shows linear response in the range from 1.0×10-6 mol·L-1-1.0×10-4mol·L-1 in 9-iorophenanthrene solution. The regression equation is I0/I-1 =0.42+1.47×104C. This method has been applied to the determination of Fe3+ with satisfactory results. Thetechnique provides a simple, effective and sensitive method to assay the Fe3+.3. The room temperature phosphorescence (RTP) of 9-bromophenanthrene (BrP) can beinduced byβ-cyclodextrin (β-CD) and micro cyclohexane (CH) in the presence of oxygen, but it is quenched obviously by trace SudanⅢ. A novel optical phosphorescence chemical sensor based on the flow injection renewable drop was established for monitoring SudanⅢ. The experimental conditions were investigated. Under the optimum conditions, the linear range of was SudanⅢ2.0×10-7mol/L to 6.0×10-5 mol/L, and the limit of detection was 0.1μg/ml. This method has been applied to the determination of SudanⅢwith satisfactory results. The technique provides a simple, effective and sensitive method to assay the SudanⅢ.
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