Research On Modification Of KGM And Application In Urease Immobilization

In this paper, the modification of KGM and apply them in urease immobilization have been researched. Concentrate on sponge KGM carrier, cross-linking KGM carrier and KGM-ALG microballoon sphere. Besides these, properties of the free urease and immobilized urease were also discussed. The characterizations by light microscope, SEM and FT-IR provide academic supports.The conduct of low temperature treatment before infusibility treatment made the carrier show an appearance of sponge and springiness. Its' properties such as merchanic intensity, storage stability and using ability are all improved. And it boasts a high sensitivity in the change of pH and has a better compatibility on substract.The cross-linking immobilized urease enjoys great storage stability, after stored in 4℃for about 30 days, its' enzyme activity appears litter lose. When we install 10.0g immobilized urease of this kind into a pillar and use it to catalyse urea-phosphate buffered solution for 3 days in run, totally catalyse 1000ml urea-phosphate buffered solution, and then sealed it with film, stored it in 4℃for around four months, and the enzyme activity remains 40%.KGM-ALG microballoon sphere immobilized urease boasts good enzyme activity over 8.9U/g. We can adjust the enzyme release rate and merchanic intensity through changing the proportion of KGM, ALG sol and the concentration of chitosan. The method is easy-conduct, mild-condition, fitting many substances' immobilization from active protein medicine, enzyme to hydrophobe, and available for massive production.