Studies On Technics And Antihyperlipidemic Effect Of Probiotics Koumiss Tablets

Koumiss(kumis,kumys,coomys,chige,airag) was a slightly alcoholic mare milk beverage,originally obtained by natural mix of starters such as lactic acid bacteria and yeast.The koumiss was rich in protein,lactose,fat,fatty acid,vitamines and many kinds of elements such as Ca,Fe,Mg,K,I,Cu,Mn,Zn.The contents of nutritions were close to that of human milk.Therefore,it could be the substitute of human milk.Drinking koumiss not only redound to assimilation but also can defend and cure many kinds of diseases such as gastroenteritic disease,cardiovascular disease, arteriosclerosis,coronary heart disease,hypertension,tuberculosis,septicemia and trace element deficiency.The results of our experiment indicated that koumiss had excellent therapeutic effect of reducing the serum lipid and cholesterol.Two kinds of methods for preparation of koumiss powder and koumiss tablets were studied in this paper.The koumiss tablet was prepared with koumiss powder, skim milk powder,aspartame(APM),amylum and carboxymethylcellulose sodium (CMC-Na).By using orthogonal design,the formula of koumiss tablets was optimized.The appearance index,tablet general index of it has been observed.The antihyperlipidemic effect was studied by animal experiment.The orthogonal experiment indicated that the influence of hardness of tablet was sifter size(C,mesh)＞magnesium stearate(B,%)＞CMC-Na(A,%).The optimized formula was A₃B₃C₂,3%CMC-Na as bond,3%Magnesium Stearate as lubricant, sifter of 20 mesh to make granule.The influence of taste of tablet was koumiss powder(A,%)＞APM(C,%)＞skim milk powder(B,%).The optimized formula was A₂B₃C₁,koumiss powder 50%,skim milk powder 15%,APM 1%.The result of quality standards show that the appearance index,tablet general index met our requirement,and the contents of lactic acid bacteria and yeast were very high.In the first method,the content of lactic acid bacteria was up to 1.65×10⁹CFU/g,that of yeast was up to 3.60×10⁸ CFU/g;In the second method,the content of lactic acid bacteria was 6.40×10⁴CFU/g,that of yeast was 4.15×10³CFU/g.The result of animal experiment indicated that the contents of serum TC,TG,LDL-c of model group were significantly higher than that of control group(P＜0.01). It meant that the hyperlipidemia models were developed successfully.The TC,TG, LDL-c of high dosage group were significantly decreased compared with those of model group(P＜0.01).The TC of low dosage group and disinfect group significantly decreased compared with that of model group(P＜0.01).The LDL-c of low dosage group and disinfect group were decreased(P＜0.05).The TG of them were decreased (P＞0.05).Therefore,koumiss powder significantly defended hyperlipidemia. The prepared koumiss tablet was rich of nutrition,convenient in taking and saving,reasonable in formula,feasible in technology and it meets the quality standards.It was a kind of sanitarian food with nutrition of koumiss and healthy function in blood lipid reduction.