| A kind of magnetic supports which can be used in the process of adsorbing plasmid has been prepared in a fast and economic way. Firstly, stable ferrofluid with Fe3O4 particles was obtained by a coprecipitation process using lauric acid and oleic acid as dispersant respectively. Secondly, the magnetic supports with epoxy group was prepared by a modified suspension polymerization process using GMA as monomer, EDMA as cross-linker, PVA as stabilizer, AIBN as initiating agent. The characters of the magnetic supports were detected by different apparatuses such as size analyzer, IR, TEM, ESEM, VSM. Then the magnetic supports modified by DEA or DEAE became anion-exchange resin which has amino groups.The result of size analyzer indicate that the supports have narrow size distribution range from 1μm to 10μm, and its'volume-weighted mean diameter is about 4.49μm. The TEM image of the ferrofluid show that it has spherical shape with an average size of 20nm, the image of microspheres'show that the supports have core-shell structure, and magnetite nanoparticals were well dispersed in the polymer matrix. VSM result show the supports'saturation magnetization is about 19.9emu/g at room temperature with little coercivity and remanence. The ESEM photograph show the supports have a spherical shape and its'size range is in accord with the result of size analyzer. The analysis of IR demonstrate there are epoxy groups on the surface of the supports, after modified with DEA, the epoxy groups dispersed, and the supports became anion-exchange resin with amino group, so they can be used in the process of adsorbing plasmid. Their adsorption capacity is about 0.635μg/mg wet resin, and can be reused with several times.
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