Research On Hydrogen Production And Influential Factors Using Straw By X9

As advanced renewable energy, hydrogen is attracted more and more attention. In most methods of producing hydrogen, using microbe to ferment biomass such as organic waste water, agriculture solid wastes and organic rubbish in anaerobic condition to produce hydrogen is becoming a noticeable problem all over the world, because of the advantage of getting rid of wastes, protecting environment and producing energy. And using low-cost agriculture organic wastes to produce hydrogen is a hot problem. While the correlative researches are very few and stay in the stage of library, because of the problem of low utility efficiency and the gain and culture of hydrogen producing bacterium.Four different pretreatment methods were applied to deal with the corn straw. Using the straw after pretreatment as the substrate, the influence of different methods to hydrogen production by X9 was discussed. The results showed that, the straw pretreated with H2SO4 solution and acidizing steam blasting was suitable for hydrogen production. After pretreated by 0.5% H2SO4 solution and acidizing steam blasting, the hydrogen yield were 18.14ml H2/g straw and 20.58ml H2/g straw respectively. The decomposing rates of straw were 9.37% of 0.5% H2SO4 solution pretreatment and 9.88% of acidizing steam blasting. The straw preteated with alkali and ammonia was not suitable for hydrogen production.Straw and eluent after acidizing steam blasting were used as fermentative substrate, the biogas and hydrogen yield were 98ml (83.8%) and 59.6ml (74.8%) than controlled trial. But the decomposing capacity of straw was not enhanced, it indicated that X9 could use other substance in eluent to product hydrogen.The results of influences of magnesium and iron on the fermentation by X9 grown on straw showed that, in the case of 100mg/L MgCl2·6H2O, the amount of hydrogen evolved was 54.8ml, which was higher than the case of 10mg/L MgCl2·6H2O obviously. The concentration of magnesium would influence the capacity of hydrogen production by X9 grown on straw. The influence of iron on X9 was not obviouse, a small quantity of iron would meet the requirement of growth and hydrogen production. And the increasing of iron concentration did not accelerate hydrogen production and straw decomposing of X9 obviously.X9 and HPB B49 were cultivated in the mixed manner to investigate the hydrogen-producing capacity. As the results showed, the hydrogen yield of mixed culture was less than that of pure culture of X9. The amount of VFAS was almost the same both of mixed culture and pure culture. It indicated that, the hydrogen-producing capacity of X9 in the mixed culture was inhibited. Meanwhile, the HPB B49 was also inhibited by X9, because the concentration of ethanol produced by B49 was very low. So, in the mixed culture, both X9 and B49 were not in their best hydrogen-producing conditions.