Real-time Quantitative PCR For Assessing The Bacteria Related To The Excess Sludge Demineralization

How to not discharging the excess sludge, which is always a major problem of sewage treatment, although there are some new crafts, we could not control the bacterial habitat well during the course of operation. The bacteria in the sludge impact on the treatment effect of the wastewater, we could improve the control capacity of the treatment system by understanding the bacterial diversity and dynamic information. The biological system of wastewater treatment system can be considered as an artificial ecosystem, which adapted to the extreme environment, just like the natural ecosystems, it has its own structure, its biological community structure also changes in time and space.Molecular biology technology makes the research on the phenomenon of life developed quickly on the molecule-level. Application of real-time quantitative PCR method, we could establish the standard curve of some functional bacteria and the total bacteria in the sludge, and get their quantity. Using this method, we could make a dynamic analysis to some specifically bacteria, which make the excess sludge demineralization.This experiment based on the gravel contact oxidation technology, taking molecular biotechnology as a method, taking the bacteria in the sludge as the object of study, and applying the real-time quantitative PCR technology to make a quantitative analysis on some functional bacteria which make the excess sludge demineralization. Applying some appropriate human approaches during the operation of the project to change the relative number of some bacteria in the reactor to improve the habit of the reactor, ultimately achieve the purpose of not discharging the remaining sludge.This experiment choose the conventional activated sludge reactor as contrast object, in order to reflect the way of bacterial working in the sludge by contrasting bacterial quantity of two actors.First of all, two reactors start-up,linked to film, operated normally ,then after a long period of stable operation, the water became stability and water quality standards (integrated wastewater discharge standard, GB 8987-1996)can be achieved. During the operation, extracting the DNA of the bacterial in the activated sludge and biofilm to do the molecular biology experiments, and making a dynamic analysis to the bacterial in the sludge.