Designs And Optimizes Of Stirring Bioreactor

To carry out the various products by the aid of cell cultivation has already been an important component of our country biotechnology, it relates to much industry such as medicine, chemical industry, light industry, food, agriculture, ocean, environmental protection. Since our country's commercializes large-scale cell reactor product is in blank space, therefore it has very big exploitation space. Bioreactor in the past is designed depending on experience mostly, in recent years, computational fluid mechanics method is used for flow and commixture characteristic property studying of the bioreactor, the theoretical analysis for bioreactor designs having provided new method.Component first introduce the basic structure of stirring bioreactor, the model making use of border condition law in computational fluid mechanics special use software FLUENT software to carry out building structure on important reactor inner component analyses according to the characteristic stirring structure of stirring bioreactor, find that by the fact that the flow distribution analyses contrast to inside of reaction boiler, the form stirring the structure stirring an awe's within bioreactor will decide the reaction boiler inner flow distribution form , the reaction boiler geometry form will affect the material transform efficiency, survival rate of living things cell then. Stir speed will affects absolute internal flow distribution speed, also be an important parameter to affect the reactor shearing.Then this component adopt the especially accurate three-dimensional moving mesh analysis way to analyze the boiler inner fluid condition, compares the data with document experiment, discover the flow distribution speed that this method gets , shear force, rapids kinetic energy meet with the data of experiment. Therefore this analyze way is able to be used to consult forecast reaction boiler inner 3-D flow distribution condition consistently. The kinetic energy analytical results demonstrated that the shearing force and velocity scale unevenly in the boiler, focuses on the propeller mainly. For improve the misdeals condition in boiler, change every critical piece structure and the dimension parameter, compare with the results, and optimize the structures. The article produces condition finally according to actual usage demand of medicine biotechnology Ltd of Harbin, carrying out selections on basic structure of bioreactor. Then reference to relevant data designs reckoning to the theory stirring bioreactor, mainly completed the wall thickness, fixed flange thickness, main bolt diameter, mixing axis diameter and so on machinery structure of bioreactor. Make one entire package method for stirring bioreactor designs.