Performance Properties And Application Of Anti-brine PAM With High Molecular Weight

This paper focused on the analyzing method of azelaic acid, the breeding of azelaic-producing strain and its characterisrics,t he shaking flask experiments,the ,atter influenced the cytochrome P450 or related enzyme as well as purification of azelaic acid.Taking Candida tropicalis TSW-1 as oriinal strain, the azelaic-producing strain, where dimorphism of thread-like was observed, was selected after ions implantation induced mutagenesis.The relations between the survival and dose of Candida tropicalis by different ions implanted dose, and the best results was obtained by 40keVN+ implantation.Titration amalysis method was set up for azelaic acid; the error was less than 1.5%.In fermentation process,dissolved oxygen of broth,concentration of urea and tridecane,concentration of tridecane,the value of pH and cultivating temperature highly affected azelaic acid productivity.When using baffled flask,optimal cultural medium was obtained,in optimal fermentation condition,strain age was 42~44h,inoculum was 15%,the value of pH was 7.5~8.0,the cultivating temperarure was 31℃,in this comdition,the yield of azelaic acid was 50.00g/L.During the fermentation process, the matter, which can influence the cytochrome P450 enzyme activitv or reated enzyme,greatly affected azelaic acid producvity too.Phenyl barbital as tridecane can induce cytochrones P450.Acylic acid and acetate,tween80,cellblock tridecane benefited the production of azelaic acid.Using new method of fermentation,by adding the sucrse or tidecane at the later circulation of fermentation,the yield of azelaic acid reached 62.00g/L.Proteins were roughly eliminated from fermented broth by alkaline and heat disposal,through filter,the straina was eliminated.Its color decreased by adding 3% activated carbon when heated.When the value of pH was decreased to2.0~3.0, white crystal of azelaic acid wsa obtained.