| Chemiluminescence has become one of the focus in the area of analytical chemistry due to its advantages such as higher sensitivity, wider linear range, simpler instrumentation, versatility of use and the easy automation. Capillary electrophoresis (CE) has gained significant acceptance in the analytical laboratory. The effciencies afforded by CE lead to high resolution separations with small sample volumes and short analysis times. Here we combined the detection method with capillary electrophoresis (CE) technique, and discussed its application for environmental and biological samples analysis. The main contents are shown as follows:1. A novel method for the analysis of biomolecules was developed by capillary zone electrophoresis-chemiluminescence (CZE-CL) method based on the fact that some amino acids and proteins could catalyze the chemiluminescence reaction of luminol-K3Fe(CN)6 system. In addition, 2.5 mmol/L sodium tetraborate- 2×10-3mol/L luminol and 2×10-4 mol/L potassium ferricyanide (pH=13.3) were chosen as optimum separation and detection condition for the analysis of L-arginine and pepsin. The detection limits (S/N=3) for L-arginine and pepsin were 1.3×10-4 mol/L and 7.1×10-7 mol/L, respectively. Finally, the presented method was used for the analysis of three real L-arginine samples successfully.2. A capillary electrophoresis(CE) with on-line inhibited chemiluminescence (CL) detection was firstly used for the simultaneous analysis of benzenediol isomers and phenol. It was based on the quenching effect of benzenediol isomers and phenol on the chemiluminescence reaction of luminol with potassium ferricyanide in sodium hydroxide medium. Under the optimum conditions, the four phenols were baseline separated and detected in less than 10 min. The detection limits (S/N=3) for hydroquinone, resorcinol, catechol and phenol were 2.9×10-8 mol/L, 3.7×10-7 mol/L, 8.4×10-8 mol/L and 4.4×10-6 mol/L, respectively. The RSDs were between 2.5～4.8% (n=5). Finally, the presented method has been successfully applied to hair dye samples and coke plant wastewater sample.
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