Preparation Of Magnetic Nano-microspheres And Their Pilote Application For Glutathione Separation

In this paper,magnetic polymer microspheres(MPM) were synthesized by using of the as-prepared Fe₃O₄-nanoparticles,and their pilote applications for glutathione separation were also studied.The detailed contents of this paper are given as following:1) Using ferric trichloride,ferrous sulfate and sodium hydroxide as main source materials, we successfully prepared the well-dispersed Fe₃O₄ nanoparticles by co-precipitation method. The as-prepared nanoparticles were characterized by XRD,TEM,FT-IR and VSM.The results showed that as-prepared Fe₃O₄ particles with an average diameter of 8.0 nm adopted face-centered-cubic(FCC) crystalline structure,and were of superparemagnetic behavior. Many factors which affected the size of Fe₃O₄ particles,such as Fe³⁺ to Fe²⁺ molar ratio, reaction temperature,reaction time and pH value were studied by an orthogonal-design method.The optimal preparation process was therefore obtained.2) In the condition of BPO as an initiator,polyethylene glycol(PEG-6000) as a dispersant, the magnetic polystyrene microspheres were synthesized via a dispersion-polymerization method on the surface of the Fe₃O₄ particles prepared above in an ethanal-water solution.The optimal process for the preparation of MPM has resulted from the studies of the influences of the ratio of ethanol to water,monomer concentration,stabilizer concentration and reaction temperature on the size and magnetic content of MPM.The as-synthesized MPM were characterized by XRD,TEM,FT-IR,TG and VSM.The results showed that the MPM particles with an average particle size of 100 nm,the content Fe₃O₄ of 14.5%and saturation magnetization of 14.4 emu/g exhibited distinct superparamagnetic characteristics at room temperature.3) The separations of glutathione(GSH) with three different magnetic carriers(MC),such as Fe₃O₄ nanopatricles(MCI),Fe₃O₄ nanoparticles chelated Cu²⁺(MCâ…¡) and Fe₃O₄ nanoparticles chelated Ni²⁺(MCâ…¢),were investigated.It could be concluded that the amounts of GSH adsorbed on MCâ…¡were in the range of 8.3～8.61 mg/g,three times that of MCâ…¢.Moreover,the adsorption of GSH on the Fe₃O₄ carriers desorbed difficultly,implying this process was irreversible.The result of paper-chromatography study indicated that GSH separated from mother liquid were mostly oxidized to glutathione oxidase(GSSG) during all three processes above.