Study On Prevention Of Mildew And Adsorption Of Mycotoxin With Nano-ZnO And Its Complexes

Moldy feed and the resulting mycotoxin contamination problem is a global problem,it has caused great Against to livestock production and human healt h.Though people have been doing many things for mold,the fodder crops are still polluted by mycotoxin in different levels when they are in the field or bei ng reped and storaged.At present,the most effective way to prevent the feed to be moldy is adding preservatives and Toxin adsorbent to the feed,Researchi n the antimicrobial agent and the adsorbent with high efficiency,low toxicity, low cost,with broad-spectrum anti-bacterial and mycotoxin adsorption capacity is the next major trends.In this paper,homogeneous precipitation method has prepared nano-ZnO as NaOH and Na₂CO₃ used to be precipitants,and we studied the calcination temperature impact of the size of nano-ZnO.Consulting the experimental results,with the increase in calcination temperature,ZnO has generated the phenomenon of sintering reunion,and the size would be larger.ZnO calcined by 300℃has the minimum size about 8.7nm.Antibacterial Experimental results show that the smaller the size of nano-ZnO,the better its anti-bacterial properties,as 300℃calcination of ZnO has the best anti-bacterial properties.Zeolite and bentonite as kinds of feed additive are used to be ZnO carrier of antimicrobial agents.With co-precipitation method we obtain zeolite-nano-zinc oxide complex.XRD characterization showed that the XRD spectra of complexes in a position 30-40°of the characteristic peaks which have been proved to be zincoxide.It shows that Nano-ZnO has been successfully loaded on the zeolite and bentonite.The experimental results show that anti-bacterial nano-ZnO loading on the zeolite as well as affect their antimicrobial properties,with zeolite and ZnO when the mass ratio of 3:1 has the best anti-bacterial properties.Zeolite as it's high temperature and acid-resistant,it is not satisfactory to be modified,so bentonite is used to be new carrier of ZnO when it had been modified,results of mycotoxin adsorption experiment by ELISA method show that bentonite-nano-zinc oxide complex mycotoxin has better adsorption capacity than the unmodified zeolite and bentonite also has a wider range of mycotoxins adsorption.