Determination Of Highly Toxic Drugs In Biological Sample By Capillary Electrophoresis And Microchip Capillary Electrophoresis

Owing to its low solvent consumption, high resolving power and short analysis time, capillary electrophoresis (CE) has become a powerful tool, and has been widely used in the analysis of highly toxic drugs and chemicals. CE has developed into a very effective research area for the analysis of biological samples gradually.In this paper, basic knowledge and the application of CE were introduced at first, then several stacking technology in CE was summarized. The research contains two parts. The first part is about the determination of barbiturates in rat plasma, we stack four barbiturates by on-line large volume sample stacking (LVSS) in capillary electrophoresis. The second part is about the determination of sodium fluoroacetate by microchip capillary electrophoresis with contactless conductivity detection.In the first part of this study, the experimental conditions were optimized, including kinds of buffer, buffer pH, methanol concentration in buffer, buffer concentration, applied voltage, electrode reverse time before separation, injection condition and extractant. Under the optimized experimental conditions, this method affords 171.7-, 169.7-, 202.7- and 169.1-fold improvement for secobarbital, amobarbital, barbital and phenobarbital, respectively. Then using this method to detect barbiturates in plasma, it proved that the sensitivity and efficiency are very high, this method can analyze trace level of barbiturates in plasma.In the second part, the experimental buffer was optimized, and we used field amplified sample stacking (FASS) method to analyze sodium fluoroacetate, this method affords 10.57-fold improvement for sodium fluoroacetate. It proved the feasibility of stacking in microchip. Then the internal standard (IS), applied voltage and cetyl trimethyl ammonium bromide (CTAB) concentration in buffer were optimized.The two experimental results above unveiled that, analyzing barbiturates in plasma by LVSS in CE can improve sensitivity and reduce detection limit; meanwhile, detecting sodium fluoroacetate by FASS can enhance the sensitivity as well. Therefore, this research is very important for the further study and holds profound signification for the detection of highly toxic drugs concentration.