Study On Bovine Milk Protein Inhibiting Adhesion Of Helicobacter Pylori

Helicobacter pylori is a gram-negative bacterium,which is involved in inducing chronic type B gastritis,peptic ulcer and gastric cancer.The adhesion to the host cell is very important for the infection of H.pylori,anti-adhesive compounds to inhibit invasion of H.pylori are effective.It has been reported that bovine milk could inhibit the adhesion of H,pylori,but the active fraction is not clear.The aim of this study is to investigate the active fraction inhibiting adhesion of H.pylori from bovine milk.In the present research,the effect of bovine milk on the adhesion of H.pylori was investigated setting the result of Haemagglutination Inhibition Assay as index,then determined and isolated the active fraction inhibing adhesion of H.pylori,and analyzed the active fraction preliminarily, using ultra filtration,gel filtration chromatography.SDS-PAGE assay,MALDI-TOF-MS and other biochemistry technology,1)Fraction inhibiting adhesion of H.pylori was investigated from bovine milk using Haemagglutination Inhibition Assay.Higher molecular weight whey protein concentrate(HWPC)was discovered that it can inhibit the haemagglutination of the bacteria.2)Gel filtration chromatography of HWPC exhibited three fractions,F₁,F₂,F₃,and only F₂ fraction showed the activity of haemagglutination inhibition.And the haemagglutination inhibition titer was 1∶16,minimum inhibitory concentration(MIC)was 2 mg/ml.3)MALDI-TOF-MS and SDS-PAGE assay of F₂ suggested the active fraction was a complex with molecular weight of 73 KDa—164 KDa,contained multimeric state of whey protein made from 14 KDa and(or)18 KDa monomers.These data presented herein,a certain state of higher molecular weight whey protein can be implicated in the activity of inhibiting adhesion of H.pylori.