Extraction Of Phycoerythrin And Treatment Of Alkali Waste Water With Membrance Separation

As the By-products of Gracilaria agar Industry, Phycoerythrin which character is similar to the polymer pigment, but we have not yet learned how to reuse them as fully as we can.This thesis is comprehensive utilization of Gracilaria algae. Using the membrane separation technology to extract phycoerythrin,agar, sugar,protein and other active substances. Recycling the wasted aqueous alkali and making the effective ingredient to be used comprehensively.Both saving water resources, eliminated the pollution and achieving the purpose of cleaning production technology. The results of the study as follows:1,Phycoerythrin extraction and separationUsing the immersion and breaking methods to extract Phycoerythrin from Gracilaria.The crude of Phycoerythrin concentrated by membrane separation technology,centrifuging, and then by the salting-out, hydroxyapatite adsorption chromatography and sephadex G-100 chromatography and other separation and purification methods, the production which purity can reach 5.0 is obtained . There are three absorption peaks, respectively at 498nm, 535nm, 565nm.2,Study on the effect of alkali-treatment during the Processing of agar from GracilariaOn the basis of single-factor, study the different conditions of alkali treatment, such as the temperature of alkali treatment, the time of alkali treatment and alkali concentration, using the orthogonal experimental to optimize the production processing of Agar from Gracilaria.The optimum conditions during the processing of Agar from gracilaria was that:the time of alkali treatment was 60 minutes, the temperature of alkali treatment was 90℃, the aqueous alkali concentration was 5%, Under the best extraction conditions, the extraction efficiency of the agar from Gracilaria was 18.8%.3,Study on the effect of acidize and bleaching technology during the processing of agar from GracilariaOn the basis of single-factor,study the factors which impact of Agar rate, such as mass fraction of chlorine, the time of bleaching,the time of acidification,the pH of acid solution, using the orthogonal experimental to optimize the acidification and bleaching technology.The optimum conditions during the acidize and bleaching technology was that: the mass fraction of chlorine was 0.11%, the time of bleaching was 6 minutes, the time of acidification was 5 minutes, the pH of acid solution was 1.28.4,Using the membrane separation technology to recovery the waste aqueous alkaliRespectively using 0.1μ, 100kDa, 50kDa, 20kDa, 5 kDa molecular weight of membrane to filtrate the aqueous alkali .Then determine the content of total sugar, reducing sugar and protein in dialysate and concentrated solution. The results showed that:Filtrated after 5kDa molecular weight of membrane, there are very small content of total sugar, reducing sugar and protein in dialysate.However, in concentrated solution the content of total sugar, reducing sugar and protein were increased significantly. Dialysate can be recycled, reused, thus reducing pollution.