Lysozyme Immobilization On Polypropylene Fiber Modified By UV Photo-grafting

Polypropylene from the 20th century, the beginning of the 60's fiber-class research and industrial production, after 90 years the rapid development has become one of the main fiber of the textile industry. However, due to the non-polar polypropylene fiber material, the lack of polar molecular chain functional group, showed a certain degree of chemical inertness, and thus also to some extent limited the scope of its application. Modification of polypropylene fiber at home and abroad on research reports, most of them concentrated in the graft on the surface hydrophilic or reactive group, while the fibers on modification of immobilized enzyme research is still rare.In this paper, in order to realize enzymatic functionalization of PP fibers, PP fibers were successively modified by UV photo-induced grafting of methyl methacrylate (MMA), amidation reaction and lysozyme immobilization. The study includes light and other factors on the MMA-gg-response effect, amidation reaction and enzyme immobilization conditions on the activity of immobilized lysozyme activity. The structures and morphologies of the modified fabrics were compared using fourier transform infrared spectroscopy (FTIR-ATR),scanning electron microscope (SEM)and atomic force microscope (AFM). Finally, we also had a preliminary study of the optical film of polypropylene grafted conducted.The result demonstrated that the optimal conditions for grafting yield on PP fiber surface were as follows: BP dosage: 0.3mol/L, according to the time pre-8min; MMA concentration of40%(v/v); UV irradiation time 60min. Modified fibers the better amidation reaction conditions was the amount of hexamethylene concentration: 30% (v/v), reaction time: 2h. When cross-linked covalently immobilized lysozyme , the amount of glutaraldehyde: 2%; lysozyme: 4mg/ml, fixed time: 10h. The effect of the grafted MMA will affect the surface amine content, and thus the activity of immobilized lysozyme have an impact, when the grafting yield was 1.69% of the lysozyme immobilized with high activity for 18U/cm2 . Modified fibers after lysozyme immobilized with good antimicrobial properties, inhibitory rate of 68.60%. By comparing before and after modified polypropylene fiber FTIR-ATR, SEM, AFM and Coomassie blue staining confirmed that MMA was grafted in polypropylene fiber surface, and modified polypropylene fiber had to achieve the immobilization of lysozyme . Photografting modified polypropylene fiber and polypropylene membrane could be immobilized laccase and immobilized laccase has some effect of decolorization and grafting yield of the immobilized laccase decolorization efficiency and impact on operational stability.