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Effect Of Heavy Metal Cadmium On Soil Microbiological Activities And Luminescent Bacteria Toxicity Test

Posted on:2010-01-07Degree:MasterType:Thesis
Country:ChinaCandidate:G H ZhangFull Text:PDF
GTID:2121360278967875Subject:Environmental Science
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1 .Cadmium contamination in soil has obvious ecological effect on soil microbes, and the effect changed with different concentrations of Cadmium and duration of pollution. When the soil was polluted by Cadmium for 2 days, the basial respiration and potential respiration of soil increased, and soil microbial biomass reduced at low concentrations, but increased in the high concentration; when the soil was contaminated for 14 days, the microbial biomass decreased, and respiration are inhibited, and respiratory activation quotient (qPR=CBR/CPR) and the pH increased with levels of pollution; comprehensive analysis, 40mg.kg-1 is a critical concentration of Cadmium pollution when the activity of soil microbes varied.2. When soil was pollutted by Cadmium, the budding potential and the budding percentage of wheat were all inhibitated, and the budding percentage was impacted greatly comparised with the budding potential. The plant height of young wheat was inhibited significantly.3. The optimal NaCl concentration for luminescence of V.ibrio fisheri is 2.2%, and the optimal NaCl range is 1.7%-3.3%.The optimum pH value is 8.0, the optimal pH range is 6.0-9.0.The luminous intensity is impacted by glucose, and the effect is decided with the glucose concentration in decision system. The logarithm phase of V.ibrio fisheri was less than 21h, the generations time is 0.95h in lofarithm phase.①The dose-effect relationship of Cadmium on V.ibrio fisheri was CUBIC curve from 0.32 mg·L-1 to 48mg·L-1 of Cadmium concentration, and the correlation coefficient between 0.92~0.96. But between 2.9~186mg·L-1 of Copper concentration, the dose-response relationship of Copper on V.ibrio fisheri is the linear, and correlation coefficient is between 0.90~0.93.②When the soil contaminated by Cadmium was extracted by hydrochloric acid(0.1M), the ectacted fluid of soil impacted luminescence of Vibrio fisheri, and the critical concentration was 10 mg·kg-1.
Keywords/Search Tags:Cadmium, soil microbial biomass, respiration, luminescent bacteria
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