| Musca domestica belongs to Insecta, Diptera and Musca domestica Division, and is an important insect resource. Their larvae are commonly known as "maggot" and have a short generation time and high reproductive ability. Its large-scale and standardized artificial breeding technology is very mature. Musca domestica protein is one of the best protein resources, because both the proporation and model of amino acids is suite for human need. In addition, because of the multiple bioactive components existed in larvae, Musca domestica has high development and application value. Previous studies have showed that Musca domestica protein contains antibacterial peptide sequence, but few researchs have related to anoxidant peptides. The Musca domestica larvae powder was taken as the research material, its hydrolyzed conditions and antioxidant peptide components as well as antioxidant ability of its hydrolyzate were studied. The research results are as follows:1. The optimal hydrolyzing enzyme was alkaline protease Alcalase and the optimal hydrolyzing conditions were: the proportion of enzyme to substrate was 3.6%; pH was 8.2; reaction temperature was 62℃; substrate concentration was 11.7% and reaction time was 3h. The DPPH·clearance rate of hydrolyzate reached to 86.39% under the optimal hydrolyzing condition.2. The scavenging and reducing ability of Musca domestica hydrolyzates for DPPH·,O2-·,·OH was in dose dependent manner and their IC50 were 3.78mg/mL,8.83mg/mL and 1.08mg/mL respectively. Hydrolyzate of Musca domestica significantly inhibited peroxidation of linoleic acid and oxidation of lard (P<0.05). The inhibited extent was in dose dependent manner.3. The hydrolyzate significantly increased the activities of SOD, GSH-PX and CAT in rat blood serum, liver and brain tissues, and significant decreased MDA level. The results demonstrated that the hydrolysate had significant antioxidant ability in vivo.4. The optimal separation conditions of hydrolysates through Sephadex G-25 were: sample concentration was 20mg/mL; optimal eluent was deionized water, elution rate was 0.5mL/min and the loading volume of samples was 1mL. Two high antioxidant components were obtained under this condition. The component 2 has higher antioxidant ability and its molecular range was 2.3KDa～1.2KDa and its DPPH·clearance rate reached 88.15%.These results demonstrated that the antioxidant ability wad closely related to its molecular weight. |
