Font Size: a A A

Design And Application Of The Fluorescence BODIPY-based Sensors For Mercury

Posted on:2011-12-04Degree:MasterType:Thesis
Country:ChinaCandidate:S WangFull Text:PDF
GTID:2121360305455611Subject:Fine chemicals
Abstract/Summary:PDF Full Text Request
Nowadays, mercury is adopted extensively in every aspect of our daily life, such as thermometers, batteries and electronic equipment. The misapplication of these products often lead to mercury leak. Other ways such as volcanic emissions, combustion of fossil fuels, especially mining, cause high concentrations of mercury in many environmental compartments and a number of human health problems. What is worse, under the methylation of microbe, it can be turned into methylmercury, which is one of the most toxic forms of mercury, causing irreversible damage to the central nervous system through the aquatic food chain.These problems have prompted the development of. methods for the detection and quantification of mercury. Recently, sensitive and selective methods for mercury analysis have been developed, propelling a remarkable growth in detection of mercury. Spectrometry, chromatography, especially hyphenated instrumental techniques, such as GC-MS, GC-ICP-MS and HPLC-ICP-MS have been exploited to detect and quantify mercury. Despite the great advances made in mercury analysis, the pace of research remains limited by the lengthy time and high cost of mercury analysis using current methods. Because of the high sensitivity and selectivity, quick response and easy signal detection, chemical sensor techniques have been developed remarkably in the field of element analysis. Hence, exploring fluorescence sensors for detection of mercury in biological and environmental samples is one of the most important research subjects in supramolecular chemistry and biomedicine.Based on the previous work in our group, an improved BODIPY fluorescent chemosensor for Hg2+ B3 was designed and synthesized with the aminophenol moiety as recogniation. In ethanol-HEPES buffer solution, B3 exhibits selective fluorescence enhancement towards Hg2+ over other metal ions and anions. The fluorescence intensities are linearly proportional to the amount of Hg2+ at ppb level in ethanol-HEPES and sub-ppb level in natural water samples. Moreover, it can detect Hg2+ in the sulfur-rich environment without interference on the fluorescence intensity. Using fluorescence microscopy, B3 is shown to be capable of imaging Hg2+ in living cells.In addition, we exploited the first sensor which can selectively detect methylmercury without interference from Hg2+. We introduced methyl palladium complexes into B3 and got the sensor B4, which have a specific identification to Hg2+ in ethanol-water through UV and fluorescence methods. Moreover, the reaction of sensor B4 with Hg2+ gave another sensor B5, which can selectively detect methylmercury in acetonitrile. It was the first methylmercury sensor.
Keywords/Search Tags:Fluorescence Sensors, Boron dipyrromethene (BODIPY) dyes, Mercury ion, Methylmercury, Natural water samples, Imaging, Living Cells
PDF Full Text Request
Related items