| With the improvement of living and cultural standards, people are increasingly pursuing healthy living. However, cancer is one of the most serious threat to human health, which has been the most difficult problem in the medical area at the present time. Researches have showed that many natural products contain anti-cancer and antioxidant components such as lycopene (LY), sulfuraphane (SF), ellagic acid (EA) and so on, which have been central issues of study. However, the application and promotion of the natural active ingridients were limited duo to their instability and poor solubility. In this paper, microcapsules, solid dispelrsions and inclusion complexes were researched to improve the stability of lycopene and sulfuraphane, what's more, to increase the solubility of ellagic in water.Lycopene was extracted from the fermentation of Blakeslea trispora using solvent extraction method. Then, the purification technology of the lycopene-rich oil resin was studied. Alkaline saponification, vacuum concentration and crystallization were performed to get the lycopene at a high purity of 90.16%. In order to improve the stability of lycopene, microcapsules and inclusion complexes of lycopene were prepared. On the one hand, the microencapsulation of lycopene was made by the method of complex oacervation with gelatin-aeaeia system. The effect of various processing parameters including wall materials, the core/wall ratio, pH, emulsifying time and stirring speed on the microencapsulation efficiency was investigated. The optimum condition was obtained by orthogonal experiment:gelatin and arabic gum (1:1) as wall material,1% HPMC as supplementary materials and emulsifiers, the core-wall ratio was 0.06:1, pH was 4.0, the emulsifying time was 20min, and the stirring speed was 400r/min. On the condition above, the lycopene microcapsules were prepared with good shape and stability. In addition, the microencapsulation efficiency was 80%. On the other hand, inclusion complex of lycopene with hydroxypropyl-β-cyclodextrin prepared successfully using the co-precipitation method with a stoichiometry 1:4. The result of the study showed that the complex is better when the inclusion time was 20h and the reaction temperature 30℃. Fourier transform infrared spectroscopy proved the formation of the inclusion complex. Besides, stability of the inclusion complex against heat, oxygen and light was greatly enhanced compared with that of lycopene.To improve the solubility and dissolution of lycopene, lycopene solid dispersions were prepared using PEG6000 and PVPk30 as carrier respectively. The result of the study showed that the solid dispersion has a higher dissolution rate when the molar ratio of lycopene to carrier in the dispersion was about 1:4.Inclusion complex of SF with hydroxypropyl-β-cyclodextrin (HP-β-CD) was prepared successfully using the co-precipitation method with a stoichiometry 1:1. The results of the study showed that stability of the inclusion complex against heat, oxygen and alkaline conditions was greatly enhanced compared with that of SF. Fourier transform infrared spectroscopy (FTIR),1H Nuclear Magnetic Resonance(1H NMR) and Ultraviolet-visible (UV) spectroscopy have proved the formation of the inclusion complex SF/HP-P-CD.Ellagic acid solid dispersions were prepared using different carriers, such as HP-β-CD, PVPk30, PEG6000, and Poloxamer 188. PVPk30 was found to be the better carrier, which can increase the solubility of ellagic acid 60 times, reaching 1.639mg/mL. Furthermore, the formation of the solid dispersions was verified by UV and IR spectroscopy.
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