Simulation Experiment Of Microbial Enhanced Oil Recovery And Oil Reduction Study By Combined Strains

The purpose of this article is to describe the research of BT-003 and BT-004 enhanced oil recovery. It includes the following three parts:the rejuvenation and growth characteristics of the two experimental strains, core experiment, the oil viscosity reduction research of combined strains.First, the species that have been rejuvenation and their growth characteristics were studied. We selected the good characteristics stains and we got the BT-003 and BT-004 stains. We also drawn bacteria growth curve to learn more about the bacteria growth process and their characteristics, the results show that:BT-003 strain got into exponential phase of growth in 2h-14h, and then entered the stable phase. BT-004 strain got into exponential phase of growth in 2h-16h, and then entered the stable phase.Secondly, in order to verify the feasibility of BT-003 strain used in the microbial enhanced oil recovery, we designed a core model based on the reservoir rock characteristics and principles of oil extraction, and applied it to the microbial enhanced oil recovery simulation core experiment. We investigated the conditions which effect the improve recovery of experiments, such as the temperature, oil content, the bacterial concentration and the closing time on the oil well, and the results show that at the temperature 40℃,30% oil content, bacterial concentration 5.5×105 cell/mL, closed well time 7d conditions, the microbial enhanced oil recovery rate as high as 22.14%. The results showed the feasibility of the BT-003 strain used in microbial enhanced oil recovery.Finally, we studied the viscosity reduction of the two combined experimental strains. The single strain BT-003 have a significant affect on crude oil viscosity reduction, from the 12000mPa·s down to 500mPa·s. BT-004 strain have slightly worse affection on the crude oil viscosity reduction than the BT-003 strain, from the 12000mPa-s down to 1500mPa·s. Although we did not get the better effect of crude oil viscosity reduction, but we can see the effect of emulsification of crude oil of combined bacteria is better than the single optimum.