Soil Enzymes And Rhizosphere Microbes Of Wetland Plants Calamagrostis Angustifolia Effect On Naphthalene Contamination

As an important component of the ecosystem of the soil microbes, involved in the complex of soil organic matter decomposition and re-synthesis, the promotion of C, N, S, P, etc. cycle. Soil enzymes is a biologically active soil protein, which in the degradation of organic pollutants and microorganisms play an important role in the process.In this study, root-box method used, the rhizosphere through nylon mesh compartment for analysis of naphthalene contamination on soil microbial indicators and indicators of soil enzymes. The results showed that:(1) Relative to non-rhizosphere soil, rhizosphere in the number and activity and soil microbial activity has increased significantly.(2) Low concentrations of naphthalene in soil pollution (the concentration of less than 50mg/kg or 100mg/kg) on some physiological indicators of Calamagrostis angustifolia has a certain stimulating effect, but high concentration of its coercive effect is obvious. Compared with the control group, 50mg/Kg and 100mg/Kg conditions can increase the concentration of the soil rhizosphere microorganisms. 200mg/Kg and the concentration of 400mg/Kg decrease in the number of soil rhizosphere microorganisms.(3) Different types of soil rhizosphere enzyme 50mg/Kg, 100mg/Kg, 200mg/Kg, 400mg/Kg different responses. Compared with the control group, 400mg/Kg under the conditions of the concentration of hydrogen peroxide in rhizosphere activity significantly reduced; 50mg/Kg and 100mg/Kg rhizosphere dehydrogenase and polyphenol oxidase activity has significantly increased, 400mg/Kg root Inter-dehydrogenase and polyphenol oxidase activity had dropped significantly; 100mg/Kg under the conditions of the concentration of rhizosphere urease activity increased, 200mg/Kg and 400mg/Kg concentrations in rhizosphere urease activity decreased significantly.(4) FDA enzyme activity in the periodⅠ(35days) andⅡ(70days) there are significant differences inⅡ(70days) activity during the FDA there is a maximum, active changes in the range of 5.4-31.9μg·g^-1·h^-1 in rhizosphere and non-rhizosphere 1.9-13.8μg·g^-1·h^-1. Study confirmed that,Ⅱ(70days) period of soil enzymes and microbial contamination of soil naphthalene critical period of adaptation and adjustment.