Study On In-situ Microbial Remediation Of Nitrate Contaminated Groundwater Simulator

Along with urban population increase and industry and agriculture rapid development, the nitrate has become the worldwide scale groundwater the most universal pollution factor, also the pollution degree increases unceasingly. Compares the alternative means, in-situ microbial remediation technology is one kind has the development potential, high efficiency and few invests environment remediation technology. This article according to the research area the model non-regular tipping site to water-bearing stratum pollution condition on the spot investigation and study and the sample analysis achievement, has conducted the technical feasibility research and the research area groundwater nitrate may the biochemistry research, explained the research area groundwater the nitrate may strengthen the indigenous microbial degradation nitrate pollution through the added-carbon source, through to studies area non-regular trash to fill in buries the field in-situ microbial remediation plan the economy and the technical contrast analysis, determined uses the advanced BAT technology (water-bearing stratum biology strengthening technology) the technical plan.The experiment carries on to many kinds of carbon sources chooses, the definite whey powder elimination nitrate effect was best, tests the 5th day nitrate most to leave for good eliminates speed vmax=34.42mg/L.d, the nitrate elimination rate r=99.92%, the reaction time and the degeneration speed already surpassed the sucrose the denitrifying effect. Through the research different density whey powder to the nitrate degeneration effect experiment indicated when is degraded the nitrate concentration is 100mg/L, the whey powder took the denitrifying carbon source the best quantity is 0.45g/L. Regardless of the whey powder all surpasses the sucrose in the processing cost and the effect, therefore the choice whey powder takes the groundwater in-situ microbial remediation carbon source. This article has also carried on the compound carbon source experiment, through the many kinds of sole carbon source combinations, the experimental result had proven whey powder + ethyl alcohol and whey powder + sodium acetate these two kind of compound carbon source elimination nitrate the effect is best, also the nitrite and the ammonia nitrogen accumulation very are also few.The in-situ microbial remediation sand trap experiment is simulates in the room which in the rocker experiment foundation carries on. In the current capacity is 12L/d, enters water nitrate 50.4mg/L, disposable throws adds whey powder 15g, the nitrate concentration assumes a small periodic variation, in which nitrate lowest concentration reduces to 2.7 mg/L, the nitrate most leaves for good eliminates rate is 95%.Throws continuously adds the carbon source the experimental result to indicate five days throw one time, each time the 10g whey powder in the sand trap base, the water leakage nitrate concentration throws a change scope three days to be bigger than, but most leaves for good eliminates rate achieves 85.5%,and falls in 12th days to minimum value 7.26mg/L.The experimental study microorganism to the nitrate degeneration effect influence, the experimental sand trap vaccination microorganism is the denitrifying bacterium which the process screens and concentrates, therefore the experiment obtains the bacterium total mainly is the denitrifying bacterium. The experiment indicated that microorganism denitrifying speed and bacterium quantity related, when carbon source sufficient, microorganism quantity and nitrate elimination rate present correlation.