| When microbial growth, they often tend to attach to the surface to form a biofilm, which is called the sediment in paper-making industry. The formation of sediment is the main reason that results in microbial contamination of the paper machine, and this problem has drawn increasing attention by more and more people. However, which kind of microbial specie(s) and how they work in the white water system has not been well known.In order to find the main microorganism which produce the microbial biofilm and then find the root causes of microbial contamination. White water of the wire pit was sampled from the PM2 of UPM(Changshu) Mill. By the conventional pure culture method, 20 dominant microbial strains were selected. Thereafter, 4 strains of these microorganisms, which were obviously different in shape, color and some other common characteristics, were used to conduct further analyses (Serial No.: N1,N2,N4,N16). The phylogenetic relationship of the 4 strains was determined by sequencing the 16S rDNA. Taxonomy of these strains was also studied through physiological and chemical tests, and morphorlogical observation. The experiments indicated: N1 and N2 were Klebsiella pneumonia, N2 was Klebsiella pneumoniae Rhinosclerom, additionally; N4 was Sphingomonas azotifigens; N16 was Enterobacter radicincitans. The thicker capsule of strain N1 and N2 comparing with strain N4 and N16 implied that N1, N2 were Klebsiella pneumonia, which likely to be the main disease bacterium that brought about pollution to biofilm of the paper machine. This study provided useful guidance for analyzing the causes for biofilm-contamination in papermaking process.As a kind of opportunistic pathogen, Klebsiella pneumonia has a widely distribution and a strong resistance, which because of its producing-enzyme and biofilms, as well as the extensive use of antibiotics These results provides a positive guide for analyze the reason of biofilm-contamination in papermaking process.
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