Study On The Preparation Of The Monolithic Bar On The Basic Of Sulfamethazine Molecular Imprinted Polymer And Its Application In Solid-phase Microextraction

Molecularly Imprinted Technology (MIT), a new method which is employed rapidly in recent years for its supplying prospective configuration in Molecularly Imprinted Polymers (MIP). When templates exist, polymerization is produced between the template and function monomer in the mixed solution. After the polymerization, the template is eluted by appropriate solvent, the template site is available which has the inherent advantages of high selectivity and solvent resistance when it is used in complicated sample.Now, Commercial solid-phase microextraction( SPME) fiber is very brittle and expensive ,also the coating materials are very limited and have the disadvantage of low selectivity, easy to get saturated and continual coating loss, it's extremely necessary to develop a novel SPME fiber with high selective, stable and high extraction efficiency.In this work, the monolithic bar on the basic of sulfamethazine molecular imprinted polymer was made with Methacrylic acid (MAA) as function monomer, Sulfamethazine (SMZ) as printed molecular , Ethylene glycol dimethacrylate (EDMA) as crossing linker, Acetonitrile as porogen and azobis-isobutryonitrile (AIBN) as the initiator. The effect of preparation condition was evaluated in order to get better imprinted site and higher extraction efficiency ,the conditions include the temperature ,the quantity of template and porogen. The MIPs were observed by scanning electron microscope (SEM) and characterized by FT-IR.Also, the monolithic bar was applied in SPME for extracting sulfamethazine. The optimal elution solution was determined and the effect of extraction time,the amount of methanol in extraction solution was also evaluated. The determination method of SMZ by SMZ-MIP-SPME-HPLC was established. The limited of detection of this method was 0.06μg/mL, with coefficient of correlation (R) was 0.9994. The extraction efficiency between SMZ-MIP-SPME the monolithic bar and NIP-SPME the monolithic bar was also compared, the former has better efficient than latter.