Research On Functional Activities Of Defatted Wheat Germ Hydrolysates

Defatted wheat germ hydrolysatess(DWGH) was producted in the factory, then it was imperative for us to know about the composition, physical property, nutritive peculiarity, and active functions of DWGH. Compared to soybean-peptides, corn- peptide and wheat protein peptide, composition, physical property and nutritive peculiarity as well as antioxidant and hypotensive activities were objectively evaluated.DWGH was equally composed of 10% moisture contents, for dry basis, 7.78 ash content, 47.42% protein content, 35.71% peptide content, 6.86% starch content, 4.86% dietary fiber , 33.77 polysaccharide content, As and Pb were not also checked. However, the protein of soybean-peptides were all above 90%, the corn-peptide and wheat protain were all above 80%, which were higher than DWGH's.The content of essential amino acid of DWGH reached 32.5%, which was less lower than corn-peptide(37.81%) and soybean-peptides(33.7%); Lysine and Leucine were lack in DWGH. The percent of relative molecular weight under 1000Da in DWGH was above 95%, and relative molecular weight between 1000Da and 2000Da took up 10.05%, the proportion of molecular weight between 180Da and 1000Da reached 66.77%, The one under 180Da was up to 19.1%.During pH 2-10, the nitrogen soluble index of DWGH was at range of 64%-71%; Oil-absorbing capacity of DWGH was 1.71ml/g; Moisture regain and moisture retaining quality was well; Below the concentration of 10%, the viscosity of DWGH was well. In vitro antioxidant assays, antioxidant activity of DWGH are best of all with respect to reducing power activity and DPPH radical scavenging activity,especially, IC50 of DPPH radical scavenging is 1.36mgdw/ml; At the concentration of 1.00mg/ml, chelating-ferrous activity of DWGH is about 40%; DWGH had good effect on inhibiting auto-oxidation of linoleic acid, which was similar with soy-bean peptides, corn-peptide and wheat protein peptide. The antioxidant activities were free of digestive enzymes. In vivo assay, DWGH significantly enhanced T-AOC and the activities of GSH-Px and SOD increased (p<0.01) significantly in the serum, increased the activities of SOD and T-AOC in heart (p<0.01) and the activity of GSH-Px in brain and liver also increased (P<0.05), meanwhile the activity of SOD in brain (P<0.05), futhermore reduced the contents of MDA in the serum and organs (P<0.05) in D-galactose-treated. So not only the DWGH is an efficient antioxidant in vitro, but also it might at least in part defer the aging process in mice induced by D-galactose.ACE inhibitory activity IC50 of DWGH was 1.04mgdw/mL, and digestive enzymes were not affected on the ACE inhibitory activity; Spontaneously hypertensivera rat(SHR) were each given 1000mg/Kg BW of DWGH by single gastrie intubation, the systolic blood pressure of rats were decreased significantly once time, 7 hour after lavaging, comparing with 0 hour, the decrease of the SBP of Captopril and 1000mg/Kg BW were respectively 12 mmHg and 20mmHg,was, which significant (P<0.05); Meanwhile, So SHRs were given DWGH at the doge of 500mg/Kg BW after one month, the systolic blood pressure of rats were decreased from 212.4±1.4 mmHg to 184.4±17.1 mmHg,which significantly changed compared with SHRs. So DWGH can inhibit ACE in vitro and decreased the systolic blood pressure of SHR in vivo.