Synthesis Of Glucose-Sensitive Hydrogel

Intelligent hydrogel is a kind of polymer material which can respond to external environment. Glucose-sensitive hydrogel is expected to be widely used in the field of clinical medicine for its distinctive property of sensitive to the changes of glucose in solution. The glucose-sensitive hydrogel that already composed almost based on the interaction mechanism between glucose and sensitive element including glucose oxidase (GOD) and plant lectins (such as Concanavalin A, ConA). But both GOD and ConA are native proteins which are very sensitive to the changing of encironment, would cause immunological reaction if exposed in vivo, unstable with short remaining time.In this dissertation copolymer hydrogel with favorable glucose sensitivity was composed, the method of synthesizing hydrogel AAPBA-DMAA-AAm, the glucose sensitivity and how to enhance this property of hydrogel were researched in detail. The main conclusions of this dissertation are summarized as follow:First, N-[3-(dimethylamino)-propyl]-N'-ethylcarbodiimide hydrochloride(EDAC) as condensing agent,3-acrylamidophenylboronic acid(AAPBA) was synthesized with PBA and AA. The product was analysed by elemental analysis, FTIR and 1H NMR, indicating the coincidence of chemical stucture with AAPBA. In order to get the maximum condensation constant, the pH of reaction solutions were adjusted in the range of 4.8-5.0. The reaction was carried out encircling with N2, whipping, ice bathed, and the product rate come up to 47%.Second, copolymerization of AAPBA with N,N-dimethylacrylamide(DMAA) and acrylamide(AAm) on the surface of glass plates resulted in the formation of a hydrogel film with porous structure. The effect of AAPBA and the crosslinking agent-MBA on hydrogel mechanical property, the effect of two different solvent on swelling degree, and the glucose-sensitive of AAPBA-DMAA-AAm hydrogel were discussed successively.The swelling degree of the obtained hydrogel was increased along with the enhancement of glucose concentration from 7.0mmol/L to 11.1mmol/L, coupling with shorter response time. The hydrogel had good sensitivity to glucose.At last, the boronate specific dye, Alizarin Red S (ARS) was incorporated into hydrogel membrane to increase the sensitivity towards glucose and poly(N-vinylpyrrolidone) (PVP) was incorporated to prevent the dye leakage. The glucose-sensitive of the colored gel was improved obviously comparing with AAPBA-DMAA-AAm without ARS.