Research On The Rapid Detection Of The Microorganisms In Green Livestock And Poultry Products

With the development of People's living standards, the proportion of livestock and poultry products in people's diet are changing ceaselessly,so the safety of quality in livestock and poultry products is causing serious problems in people's great attention. The quality of livestock and poultry products is bringing great impact to people's health and life, Food-borne pathogen is one of the main causes of food safety, Detection of food-borne pathogens is the key link in food-borne diseases prevention and control. So the microorganism inspection method of livestock and poultry products becomes a very important topic. In the world, salmonella and bacterium are main causes in bacterial food poisoning, the traditional detection is operating trival, time-consuming, It can not match the fast food poisoning diagnosis and the real-time monitoring of food safety.This article is about some researchs in rapid detection methods of salmonella and bacterium. It is divided into four chapters altogether.The first chapter is about the summmarizing of the current situation of livestock and poultry products in our country, the quality and safety of livestock and poultry products. and the progress of salmonella and coliforms detection methods.In the second chapter, the rapid detection method of coliforms was illustrated in the green livestock and poultry products, the specificity and repeatability were tested by the coliforms PetrifilmTM test pieces, compared with the traditional method, the experimental results showed that PetrifilmTM coliforms test pieces for determination of green livestock and poultry products can dispense with a lot of preparations and ending works. The operation is simple and fast, the testing results had no difference with the GB, It can be used in the test of coliforms in green livestock and poultry products.The third chapter evaluated the ELISA kit for Salmonella rapid detection.The detection of Samonella by ELISA kit and the standard method of China were compared.The results showed that the veracity of the kit was 100%.The sensitivity was 1 cfu/25g, and the specificity for non-salmonella strains was 100%, too. By tests of 90 poultry meat and 90 porks, it showed that the sensitivity rate and the specificity rate were 99.3% and 100%, respectively.The fourth chapter found the method of rapid detection of salmonella by polymerase chain reaction (PCR). Five kinds of enrichment medium were compared, and the selenite-cystine (SC medium) was selected as the best one for enrichment and PCR methods. Primers were designed, by lots of Salmonella and non-salmonella isolations, the primers had high specificity. The specificity, sensitivity, anti-interference and reproducibility were evaluated for the detection of samonella, too. The experiments showed that the PCR method was specific, sensitive, and reproducible, so it would have more application in the field of the detection of samonella.