| Phosphatidylserine (phosphatidylserine, referred to as PS) is a particularly important phospholipids, which can affect the transmission of chemical information and help brain cells storing and retrieving data, therefore, recent years it is known as "brain specific nutrients". In addition, PS can also be used in food, pharmaceutical, cosmetics industries and so on. Because these industries are in need of highly pure PS, but the content of PS of phosphatidylinositol fractions in animals and plants are very little. Therefore, people demand highly pure PS more urgentiy than before. Currently, due to the domestic technical and financial conditions are restricted and the research methods of the preparation of highly pure PS are still lag phase. So the applictions of PS to food, medicine and other industry will be restricted.In this thesis, in order to promote and expand the application of PS in food and pharmaceutical industries and provide the industrialized production with theoretical basis, I intend to research on the Silica gel column chromatography used to purify PS with enzymatic synthesis of PS as raw material. The contents of this thesis include:1. First, the enzymatic synthesis of PS as raw material and operating conditions of chromatographic separation conditions were studied, the initial screening by TLC method eluent system n-hexane-isopropyl alcohol-acetic acid; then to the separation and the PS Purity of the indexes, TLC method and gas chromatography method are combined to determine the best ratio of n-hexane elution agent:isopropanol:acetic acid= 32:59:8.45 (V/ V/V). the effects of flow rate, sample volume and sample concentration on the separation PS was studied, chromatography and determined the optimal conditions PS:The flow rate was 2mL/min, the best on the sample volume was 0.08g of crude PS/g silica, sample concentration 0.06g/mL,200-300 purpose of macroporous adsorbentâ…¡, silica gel,85%purity of the product. Last investigated the reuse of the silica gel column, the experimental results obtained silica gel column can be reused. 2. PS and PC on the adsorbent on the adsorption equilibrium and adsorption kinetics were studied and discussed, drew the following conclusions:â‘ kinetic analysis of adsorption, silica gel adsorption rate of PS and PC exponential curve, compared with a Kinetics, a rate equation can be Lagergren adsorption rate.â‘¡The pore diffusion equation adsorbent adsorption rate of PS and PC data were fitted, the correlation coefficients are greater than 0.99, indicating that pore diffusion is mainly controlled by the adsorption step.â‘¢In the adsorption experiments, the application of Langmuir model can better describe the PS and PC in the adsorbent on the adsorption equilibrium relationship.3. According to the fluid flow within the state of the column and the liquid-solid two-mass and energy transfer accompanied by a thermodynamic and kinetic mechanisms, the column adsorption PS mathematical model was establised. Two components were determined by experimenting PS and PC in a different flow rate, concentration, bed height and particle size on breakthrough curves, using the mathematical model obtained fitting parameters related to further adsorption of PS on the silica gel column. The process was studied, by investigating a different flow rate, sample concentration, bed height, bed diameter and particle size on the flow curves, and application of the mathematical model was verified, the results show that the mathematical model simulation results of different operating conditions is satisfied, that model can well describe the PS and PC in the two-component adsorption in the silica gel column chromatography process,for industrial production of large quantities of amplification and the high purity these provide some PS theoretical basis and practical guidance.
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