The Application Of Multifunctional Nanoparticles On Magnetic Resonance Imaging And Their Use For Cancer Cell Detection

From the sixties in the last twentieth century, nanotechnology has experienced fast development in the short time. It means researching the features of the matter within the nanoscale(1～100nm),and use the multidisciplinary science or technology of the feature. It makes people know and reform the world expanding to the level of atom and molecule.And that nanometerial is researched from eighties in the twentieth century,combining the nanometerial with life science which we call nanobiology technology is a hot research subject among scientists,while nanometerial plays an important part in magnetic resonance imaging.For its strong function,no damaged property and providing perfect image dissecting,it becomes very popular among doctors and necessary tools of clinic diagnose.In recent years,as the fast development of nanotechnology,kinds of contrast agents show up continually,it adds new strength to the development of magnetic resonance imaging technology. Because of the advantage of superparamagnetism iron oxide nanoparticles on small size,superparamagnetic, uniform, stable and biocompatible , it becomes a good contrast agent for researchers to choose in medical magnetic resonance imaging. Furthermore, nanometerial has good magnetics properties, it shows great advantage on cells magnetic separation. By external magnetic field, modified functional magnetic nannoparticles can quickly and efficiently collect fewer caner cells in blood cells,then get the convenient way to detect. This article mainly researches the application of multifunctional magnetic nanoparticles as a contrast agent on medical magnetic resonance imaging of cancer cells ,and its exploration the condition of collecting and detecting fewer caner cells in blood cells. (1) Using chemical coprecipitation method to prepare dextran-coated magnetic nanoparticles,by doing the test technology of XRD, IR,TEM,VSM to characterize the particles. Using the chemical bond to connect biomolecule antibody on the surface of the particles. then mark the lung cancer cells, The staining method of Prussia Blue and T2 decline percent about MRI assay antibody linked efficiency respectively. MTT tests the cells viability after multifunctional immunoparticles enter cells 48h under lower concentration. It comes to a conclusion that there is no big effect on cells viability. Then use a series of lower concentration of immunoparticles entering the cells, doing MRI test, it is concluded thatγ-Fe2O3 magnetic nanoparticles can be well used as a target contrast agent on lung cancer cells under lower concentration.(2)The silica-coated superparamagnetic nanoparticles were synthesized via the reverse micro emulsion method. The surface of the nanoparticles was modified with amino group. Via the action of link-crossing agent to connect biomolecule targeted on the cancer cells, by magnetic separation setting up an efficient and fast method to collect fewer cancer cells in blood cells,the limit of detection can reach enriching about 25 cancer cells in 50 cancer cells .The efficience of enrichment is about 50 percent.we believe it provides a quick and sensitive method for cancer detection.